Skip to main content
Fig. 3 | Arthritis Research & Therapy

Fig. 3

From: Implementation of automated behavior metrics to evaluate voluntary wheel running effects on inflammatory-erosive arthritis and interstitial lung disease in TNF-Tg mice

Fig. 3

Inflammatory-erosive arthritis and generalized osteopenia are exacerbated by voluntary wheel running in TNF-Tg mice. Representative images of ankle joints by micro-CT are provided for each group, with the talus identified by color (A–H). Talus bone volumes were significantly dependent on and reduced with activity in male mice (I), but activity demonstrated no effect on female talus bone volumes (J). Qualitative assessment of representative H&E-OG stained ankle joints showed a remarkable increase in synovial inflammation (purple) surrounding the talus (arrows) with running activity for both male (K, L) and female (M, N) TNF-Tg mice. In addition, subchondral bone metrics in the femur (bone volume per total volume = BV/TV, trabecular thickness = Tb.Th, trabecular number = Tb.N, trabecular separation = Tb.Sp) demonstrated reduced BV/TV and Tb.Th with running activity in male mice (O–R). On the other hand, female mice (S–V) showed no change in subchondral bone metrics related to activity. Statistics: 3-way mixed-effects (J) and 2-way ANOVA (I, O–V); *p<0.05, ***p<0.001, ****p<0.0001. Black scale bars = 500μm (K–N)

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us