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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: TRPA1 as a potential factor and drug target in scleroderma: dermal fibrosis and alternative macrophage activation are attenuated in TRPA1-deficient mice in bleomycin-induced experimental model of scleroderma

Fig. 2

Bleomycin-induced collagen accumulation was attenuated in the skin of TRPA1-deficient mice. Representative images of Masson’s trichrome-stained sections of mouse skin harvested after 28 days of treatment with bleomycin or from control mice (A). Graphical representation of collagen measurement (B). Collagen content was measured using the ImageJ software. Col1A1 (C), Col1A2 (D), and Col3A1 (E) mRNA expression in the skin from WT and TRPA1-deficient (TRPA−/−) mice treated with bleomycin and in corresponding controls were measured by RT-PCR and normalized against GAPDH mRNA. WT control was given the value of 1 and the other values are set in the proportion to that value. Bars represent means of the collagen content + SEM; n  =  6. *p < 0.05, **p < 0.01 between bleomycin treated WT and TRPA1-deficient mice; ###p < 0.001 and #p < 0.05 between control and bleomycin treated mice within the same genotype

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Arthritis Research & Therapy

ISSN: 1478-6362

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