Skip to main content
Fig. 6 | Arthritis Research & Therapy

Fig. 6

From: TRPA1 as a potential factor and drug target in scleroderma: dermal fibrosis and alternative macrophage activation are attenuated in TRPA1-deficient mice in bleomycin-induced experimental model of scleroderma

Fig. 6

IL-4-induced expression of PPARγ was enhanced by bleomycin; no effect on STAT6 phosphorylation. J774 cells were incubated with bleomycin and/or IL-4 for the indicated time. In A: PPARγ mRNA levels were measured with RT-PCR and normalized to GAPDH mRNA. Untreated control cells were given the value of 1 and other values are set in the proportion to that value. Data are expressed as mean + SEM, n = 4. *p < 0.05, **p < 0.01, ***p < 0.001 vs. untreated control cells (vehicle group) in the same time point. ##p < 0.01 between the marked pair of values (A). In B: pSTAT6 protein levels were assessed using Western blot and normalized to total STAT6. pSTAT6/total STAT6 ratio in the IL-4-treated cells at each timepoint was set as 100% and the other values are presented in relation to that value. Bleomycin had no statistically significant effects when added on top of IL-4

Back to article page

Arthritis Research & Therapy

ISSN: 1478-6362

Contact us