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Fig. 2 | Arthritis Research & Therapy

Fig. 2

From: Identification of anti-citrullinated osteopontin antibodies and increased inflammatory response by enhancement of osteopontin binding to fibroblast-like synoviocytes in rheumatoid arthritis

Fig. 2

IgG from RA patients’ sera with anti-cit-OPN autoantibodies enhanced binding of fibroblast-like synoviocyte (FLSs) with OPN. A The 96-well plates were coated with OPN. FLSs were transferred to protein-coated plates and incubated at 37 °C for 120 min with pooled IgG from anti-cit-OPN positive RA patients or anti-cit-OPN negative RA patients. After washing, the number of cells was estimated using a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. B Quantification of the five different donors. Data are shown as mean ± SEM from the aggregate data. * p < 0.05 and ** p < 0.01 by Tukey–Kramer test

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