Fig. 1

MYC was upregulated in an osteogenic cell model and hip ligaments in patients with AS. a Fibroblasts from OA (n = 3) and AS (n = 4) patients were cultured in osteogenic differentiation medium (ODM) or control medium (CON). Stem cell transcription factors were measured by q-PCR on D10 and D20. b Upregulated transcription factors were assessed by q-PCR in ligaments from OA (n = 12) and AS (n = 12) patients. c Transcription factor MYC was measured by WB in ligaments from OA (n = 4) and AS (n = 4) patients. GAPDH was used as the housekeeping gene. The grouping of gels/blots was cropped from different parts of the same gel. No high-contrast (overexposure) of blots was performed. d Representative images shown MYC staining in the ligaments of patients with OA (n = 4) and AS (n = 3). Boxed areas in the left panels are shown at higher magnification in the right panels. Scale bar = 200 µm (left panels). Scale bar = 100 µm (right panels). Values are reported as the mean ± SEM. *p < 0.05