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Table 2 List of forward and reverse primers for PCR-RFLP of rs2277680 and rs2279115 and their associated restriction enzymes and DNA fragments

From: Metformin attenuates symptoms of osteoarthritis: role of genetic diversity of Bcl2 and CXCL16 in OA

Polymorphism

Primer sequence (5′-3′)

TA (°C)

Restriction enzyme

DNA fragment size (bp)

References

938C>A (rs2279115)

F-CTGCCTTCATTTATCCAGCA

R-GGCGGCAGATGAATTACAA

58 °C

BccI

37°C/1h

300/189/111

[1]

A181V (rs2277680)

F-CAGAAGCATTTACTTCCTACCAG

R-ACTGTGGCTGATGTCCTGGCT

64 °C

PvuII

37°C/16h

281/206/75

[2]

  1. TA annealing temperature, bp base pair
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Arthritis Research & Therapy

ISSN: 1478-6362

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