Fig. 3From: TAK-715 alleviated IL-1β-induced apoptosis and ECM degradation in nucleus pulposus cells and attenuated intervertebral disc degeneration ex vivo and in vivoTAK-715 alleviated IL-1β-induced inflammation in NPCs. NPCs were preincubated with TAK-715 for 2 h, and then, IL-1β was added for 48 h. A Western blot analysis was used to analyze COX-2 and HMGB1 in NPCs; B, C COX-2 and HMGB1 immunoblots were quantitatively analyzed. D The fluorescence intensity of COX-2 was analyzed by immunofluorescence assays (scale bar: 20 μm). E ImageJ was used to analyze the fluorescence intensity of COX-2. The data are presented as the mean ± SD (*P < 0.05; **P < 0.01; ***P < 0.001)Back to article page