Fig. 5

FTO inhibited LPS-induced chondrocyte damage through downregulating miR-3591-5p. Chondrocytes were transfected with FTO overexpression adenovirus or a combination of FTO overexpression adenovirus and miR-3951 mimics and then treated with 40 ng/mL LPS for 24 h. A The expression of miR-3591-5p was assessed by RT-qPCR. B The mRNA and protein expression of PRKAA2 was measured by both RT-qPCR and western blotting. C The caspase-3 activity was evaluated by caspase-3 activity kit. D The apoptosis of chondrocytes was evaluated by flow cytometry. E CCK-8 was detected the viability of chondrocytes. F EDU staining was used to assess the proliferation ability of chondrocytes. G Western blotting was applied to detect the expression of MMP13, ADAMTS5, Aggrecan, and COL2A1. LPS, lipopolysaccharide; miR mim., miR-3591-5p mimics adenovirus; FTO, FTO overexpression adenovirus; n = 3, *p < 0.05, **p < 0.01, ***p < 0.001