Fig. 3

MiR-325-3p was the most down-regulated microRNA in mechanical stress-treated chondrocytes and decreased in standing mice model. A Volcano plot of differential microRNAs in mouse chondrocytes with and without mechanical stress (0.5 Hz, 20% elongation, 24 h). B In situ hybridization of miR-325-3p (red) and cell nucleus (blue) staining of sham and standing 10 weeks mice. Scale bar = 50 μm. C Quantification of the percentage of miR-325-3p positive cells in cartilage area in B. n = 6 per group. All data are shown as the mean ± standard deviation (SD). **P < 0.01