Fig. 1

Effects of inhibition of Notch signaling on TLR4-mediated increases of CCL2 in DRG cell cultures. A NICD IF staining of the DRG cells treated with LPS (1 μg/mL) for 24 h. Scale bars, 50 μm. B-D Effects of inhibiting Notch signaling by DAPT (25 μM) on LPS-induced NICD (quantified as MFI and normalized to the control without LPS and DAPT treatment) (B), Ccl2 mRNA (C), and CCL2 protein (D) in cultured DRG cells (N = 4–8 cultures per treatment group). E–G Effects of inhibiting Notch signaling by sJag1 (40 μM) on LPS-increased NICD MFI (E), Ccl2 mRNA (F), and CCL2 protein (G) (N = 4–9 cultures per group)