Fig. 8

Intra-articular injection of DAPT reduces DMM-induced knee hyperalgesia and attenuates CCL2 expression in ipsilateral DRG. A Knee hyperalgesia 24-h time-course tests after IA injection of DAPT (100 μg) or vehicle in mice 12 weeks after DMM surgery (N = 6 mice per group). 450 g is the threshold baseline for naïve mice (dashed line). Area under the curve (AUC) was calculated for 0–6 h to assess knee hyperalgesia. B Ccl2 mRNA expression in the ipsilateral L3-L5 DRG from mice 12 weeks post-surgery and 6 h after IA injection of DAPT or vehicle (N = 6 mice per group). C, D Protein levels of CCL2 (C) and NICD (D) in the pooled L3-L5 DRG tissue lysates of the DMM mice 24 h after DAPT injection (N = 7 or 5 mice per group)