Fig. 3

ENT1^−/− mice show reductions in voluntary rearing without overall changes in locomotor capacity. In the open field assay, baseline voluntary locomotion was assessed over 5 min. All data are stratified by sex, each animal is represented as a unique symbol, and data are presented as mean ± SEM (n = 10 female, 10 male per genotype). A Total distance traveled in meters. One female wild-type mouse at 4 months was excluded as an outlier, based on Grubb’s two-sided test (α = 0.01). B Vertical activity count measured by disruption of the Z-plane infrared sensor. One male ENT1^−/− mouse at 4 months was excluded as an outlier, based on Grubb’s two-sided test (α = 0.01). C, D The effect of axial stretch on voluntary physical function was measured over five minutes immediately following the tail suspension assay. A difference score was calculated as the change in activity pre- and post-tail suspension (Δ = post – pre). E In the rotarod assay, locomotor capacity was quantified by the time to fall for animals on an accelerating rotating rod. Data from two male wild-type mice at 6 months of age are missing due to non-compliance with testing. *P < 0.05 by two-way-ANOVA or mixed-effects model with Sidak’s multiple comparisons test