Fig. 4

ENT1^−/− mice show reduced physical function in the flexmaze assay. A–C In the flexmaze assay, ambulation is measured as the number of double gates crossed per minute during the 20-minute test. Data are stratified by sex, each animal is represented as a unique symbol, and data are presented as means ± SEM (n = 10 female, 10 male per genotype). A The total number of double gates crossed during the full duration of the test for both sexes. *P < 0.05 by two-way-ANOVA with Sidak’s multiple comparisons test. B, C The 20 min test was segmented into five-minute intervals (0 to 5, 5 to 10, 10 to 15, and 15 to 20 min). Data are expressed as means ± SEM and are fitted with linear regressions. *P < 0.05 by unpaired, two-tailed t-test for differences between sex-matched wild-type and ENT1^−/− mice at each interval