Osteopontin - a biomarker for organ damage in paediatric lupus?

A growing body of literature has demonstrated that the multifunctional glycoprotein osteopontin (OPN), has a role in type I interferon (IFN) production and may be involved in systemic lupus erythematosus (SLE) pathogenesis. A recent report has provided further support for this, demonstrating an association between raised baseline circulating plasma osteopontin (cOPN) levels and IFN, and an elevation in cOPN prior to the onset of both increased cumulative disease and end-organ damage. These associations were most marked in paediatric SLE, implying that cOPN may be a useful biomarker of disease activity in childhood lupus.

Th e recent report by Rullo and colleagues [1] suggesting that elevated circulating plasma osteopontin (cOPN) may herald the onset of end-organ damage in paediatric systemic lupus erythematosus (pSLE) is of great interest. Th is is both in terms of highlighting a potential pSLE biomarker and to further strengthen the association of osteopontin (OPN) with type I interferon (IFN) induction and lupus pathogenesis.
Robust and reliable biomarkers for SLE, and pSLE in particular, are much needed, particularly as there are fewer validated disease markers in paediatric compared with adult SLE. Th is is despite pSLE, which represents 10 to 20% of all cases, being often more severe, with an increased likelihood of signifi cant complications and multi-organ involvement [2]. Indeed, in the present study, 70% of the pSLE cohort had renal disease at recruit ment, compared to only 17% in the adult SLE cohort [1].
Rullo and colleagues [1] demonstrated that increased cOPN at baseline was associated with antibodies to double-stranded DNA (P = 0.001), elevated IFN alpha (P = 0.02) and an increased cumulative prednisolone exposure in pSLE (P = 0.01). A longitudinal prospective evaluation of the 42 pSLE and 23 adult SLE cases was then undertaken, which showed a positive correlation between cOPN and adjusted-mean SLE disease activity index at 6 months in both the adult SLE and pSLE cohorts. Furthermore, in pSLE at the fi nal (one year) time point cOPN was signifi cantly (P = 0.001) associated with the System Lupus International Collaborating Clinics/ American College of Rheumatology damage index, thus suggesting that elevated cOPN, in pSLE especially, may be a useful biomarker to signal the onset of increased disease activity and end organ damage.
OPN exists in both a secreted (sOPN) and an intracellular form (iOPN), which are produced by alternative translation initiation sites. OPN, particularly sOPN, has many reported functions and these are regulated by posttranslational modifi cations, particularly phosphorylation. Described functions include roles in cell adhesion, diff erentiation and migration, and in the regulation of apoptosis. Importantly, when considering autoimmune disease, OPN is expressed in a number of immune cells, particularly T cells, macrophages and dendritic cells [3][4][5].
Th e work of Cantor and colleagues [6,7] is most relevant to the demonstrated association between elevated IFN alpha and cOPN, as they described links between iOPN and IFN metabolism. In murine plasmacytoid dendritic cells, the authors demonstrated that iOPN was pivotal to IFN production following TLR9 induction with CpG [6]. Most recently, they illustrated that an IFNmediated inhibition of iOPN in dendritic cells resulted in reduced pro-infl ammatory Th 17 responses [7].
Th e relationship between OPN, IFN and pSLE is also illustrated by genetic studies. Polymorphisms in spp1, which encodes OPN, are associated with elevated IFN alpha induction in young people with SLE [8]. Furthermore, pSLE and elevated levels of IFN alpha are observed in the rare Mendelian disorder spondylo enchondrodysplasia, which occurs due to a defi ciency of tartrateresistant acid phosphatase (TRAP) [9]. Interest ingly,

Abstract
A growing body of literature has demonstrated that the multifunctional glycoprotein osteopontin (OPN), has a role in type I interferon (IFN) production and may be involved in systemic lupus erythematosus (SLE) pathogenesis. A recent report has provided further support for this, demonstrating an association between raised baseline circulating plasma osteopontin (cOPN) levels and IFN, and an elevation in cOPN prior to the onset of both increased cumulative disease and endorgan damage. These associations were most marked in paediatric SLE, implying that cOPN may be a useful biomarker of disease activity in childhood lupus.

© 2010 BioMed Central Ltd
Osteopontin -a biomarker for organ damage in paediatric lupus? these patients have elevated levels of urinary phos phorylated OPN [10], perhaps indicating that a build up of activated phosphorylated OPN (due to an absence of TRAP de-phosphorylation) is responsible for the IFN signature and autoimmune phenotype observed.
Th e work of Rullo and colleagues [1] raises a number of potentially interesting questions that warrant further study. A larger scale investigation over a prolonged period is required to determine whether OPN can be utilized as a predictor of adverse disease outcome in SLE. In addition, this investigation further emphasises the need to determine the exact role of OPN in lupus, particularly pSLE, as this may be a future therapeutic target.