Toll-like receptor 2 (TLR2) induces migration and invasive mechanisms in rheumatoid arthritis

Introduction This study investigates the role of Toll-like receptor 2 (TLR2) in the regulation of migratory and invasive mechanisms in rheumatoid arthritis (RA). Methods Invasion, migration, matrix metalloproteinase (MMP)-1, -3 and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) expression, β-integrin binding, cytoskeletal rearrangement and Ras-related C3 botulinum toxin substrate 1 (Rac1) activation in response to a TLR2-ligand, Pam3CSK4 (1 μg/ml), in ex vivo RA synovial tissue explants, primary RA synovial fibroblasts (RASFC) and microvascular endothelial cells (HMVEC) were assessed by Transwell Matrigel™ invasion chambers, enzyme-linked immunosorbent assay (ELISA), multiplex adhesion binding assay, reverse transcription polymerase chain reaction (RT-PCR), F-actin immunofluorescent staining, matrigel synovial outgrowths, Rac1 pull-down assays/Western blot and zymography. β1-integrin expression in RA/control synovial tissue was assessed by immunohistology. The effect of Pam3CSK4 on cell migration, invasion, MMP-3 and Rac1 activation was examined in the presence or absence of anti-β1-integrin (10 μg/ml) or anti-IgG control (10 μg/ml). The effect of an anti-TLR-2 mAb (OPN301)(1 μg/ml) or immunoglobulin G (IgG) control (1 μg/ml) on RASFC migration and RA synovial tissue MMP activity was assessed by wound assays, ELISA and zymography. Results Pam3CSK4 significantly induced cell migration, invasion, MMP-1, MMP-3, MMP-2 and MMP-9 expression and induced the MMP-1/TIMP-3 and MMP-3/TIMP-3 ratio in RASFC and explants (p <0.05). β1-integrin expression was significantly higher in RA synovial tissue compared to controls (p <0.05). Pam3CSK4 specifically induced β1-integrin binding in RASFC (p <0.05), with no effect observed for β2-4, β6, αvβ5 or α5β1. Pam3CSK4 increased β1-integrin mRNA expression, Rac1 activation, RASFC outgrowths and altered cytoskeletal dynamic through induction of filopodia formation. Pam3CSK4-regulated cell migration and invasion processes, but not MMP-3, were inhibited in the presence of anti-β1-integrin (p <0.05), with no effect observed for anti-IgG control. Furthermore, anti-β1-integrin inhibited Pam3CSK4-induced Rac1 activation. Finally, blockade of TLR2 with OPN301 significantly decreased spontaneous release of MMP-3, MMP-2 and MMP-9 and increased TIMP-3 secretion from RA synovial explant cultures (p <0.05). Incubation of RASFC with OPN301 RA ex vivo conditioned media inhibited migration and invasion compared to IgG control. Conclusions TLR2 activation induces migrational and invasive mechanisms, which are critically involved in the pathogenesis of RA, suggesting TLR2 as a potential therapeutic target for the treatment of RA. Electronic supplementary material The online version of this article (doi:10.1186/s13075-015-0664-8) contains supplementary material, which is available to authorized users.


Introduction
Rheumatoid arthritis (RA) is a chronic progressive autoimmune disease, characterised by synovial proliferation, neovascularisation and leucocyte extravasation, joint destruction and functional disability. RA synoviocytes manifest an abnormal phenotype with increased proliferation, resistance to apoptosis and invasiveness of adjacent tissue [1][2][3]. This results in synoviocyte hyperplasia, which transforms the synovial membrane (SM) into an aggressive, tumour-like tissue 'pannus' , which is capable of destroying adjacent articular cartilage and bone.
Toll-like receptors (TLRs) have been implicated in the pathogenesis of RA with studies showing increased TLR2 and TLR4 expression in the perivascular regions of the joint, [4] at the sites of attachment and invasion into cartilage/bone, and on synovial macrophages [5]. Bacterial peptidoglycan (PG), a TLR2 ligand, has been detected in RA synovial fluids [6]. Increased expression of TLR2 has been demonstrated in collagen-induced arthritis, and streptococcal cell wall (SCW)-induced arthritis does not develop in TLR2-deficient mice [7]. Furthermore, studies have demonstrated that dominant negative forms of the TLR2 adapter molecules myeloid differentiation primary response gene 88 (MyD88) and MAL/TIRAP inhibit pro-inflammatory cytokine production in RA synoviocytes [8]. Recent data has shown that the pattern-recognition receptor nucleotide-binding oligomerisation domain-containing protein 1 (NOD1) synergises with TLR2 in the induction of cytokines in RA synovial fibroblast cells (RASFC) [9]. TLR2-induced cytokine expression has also been shown to be mediated by miR-19 in RASFC [10]. Furthermore, studies have shown the involvement of the Tie-2 receptor in mediating TLR2-induced angiogenesis [11]. Together these studies suggest that TLR2 is a key mediator involved in promoting cell migrational, invasive and destructive mechanisms in the RA joint.
Cell migration is essential for tissue infiltration during the inflammatory process and is initiated by cell polarisation and the formation of membrane protrusions at the leading edge [12]. Integrin-linked activation of the small guanosine triphosphates (GTPases) of the Rho family (Cdc42, Rho and Rac1) mediates cytoskeletal dynamics such as filopodia and lamellipodia formation and stress fibre formation, which is essential for cell migration [13,14]. Integrin-linked activation of Rho GTPases mediates gene transcription, cell cycle progression and adhesion [13,15]. Previous studies have demonstrated that integrin blockade can inhibit fibroblast invasion and adhesion of cells to the extracellular matrix (ECM) [16,17]. In particular, blockade of α2β1 integrin in RA protects against cartilage destruction via reduction of matrix metalloproteinase (MMP)-3 [18]. In this study we investigate the effect of TLR2 on migrational and invasive mechanisms in RA. We demonstrate that activation of TLR2 induces RASFC migration, invasion and induction of MMPs. Furthermore, we show that TLR2-induced migration/invasion is partly mediated by β1 integrin and downstream alterations in cytoskeletal dynamics and Rac1 activation. Finally, using an ex vivo RA synovial explant model and RASFC, we demonstrated that an anti-TLR2 antibody (OPN301) significantly inhibited MMP expression and migration. Together this study further demonstrates that TLR2 activation plays a key role in regulating invasive mechanisms in RA, a key process involved in the pathogenesis of RA.

Patients and RA synovial tissue
Patients with RA were recruited from the outpatient clinics at the Department of Rheumatology, St. Vincent's University Hospital (SVUH). Only patients with an actively inflamed knee joint and fulfilling the revised American College of Rheumatology criteria were recruited [19]. RA synovial tissue was obtained at arthroscopy under direct visualisation as previously described [20]. Normal synovial tissue was obtained from ten patients (eight men; two women) undergoing interventional arthroscopy for cruciate ligament tears. Fully informed written consent was obtained from each patient prior to inclusion. This study was approved by the SVUH Ethics and Medical Research Committee.

Immunohistochemistry
Synovial biopsies obtained at arthroscopy were snap frozen in OCT compound and stored at −80°C. Seven-micronthick sections were cut using a cryostat and placed on glass slides coated with 2 % 3-amino-propyl-triethoxy-silane (Sigma-Aldrich Ireland Ltd, Dublin, Ireland), dried overnight at room temperature and stored at −80°C. To examine β1-integrin expression and localised distribution in the synovium, immunohistochemical analysis was performed in RA (n = 17) and normal synovial tissue (n = 9). Tissue sections were fixed in acetone for 10 min and air-dried. Non-specific binding was blocked using 10 % casein buffer. A routine three-stage immunoperoxidase labelling technique incorporating avidin-biotin-immunoperoxidase complex (Dako, Glostrup, Denmark) was used. Sections were incubated with mouse polyclonal anti-β1-integrin (R&D Systems, Cambridge, UK) at room temperature for 1 h. Sections were also incubated with an appropriate isotype-matched mouse-immunoglobulin G (IgG) control antibody (R&D Systems, Cambridge, UK) as negative controls. Colour was developed in solution containing diaminobenzadine-tetrahydrochloride (Sigma-Aldrich), 0.5 % H 2 O 2 in phosphate-buffered saline (PBS) buffer (pH 7.6). Slides were counterstained with haematoxylin (BDH Laboratory Supplies, Poole, UK) and hydration and fixation was performed through a series of IMS and xylene solutions. Sections were mounted using DPX mountant (BDH Laboratory Supplies). Images were captured using Olympus DP50 light microscope and analysis software (Soft Imaging System Corporation, Lakewood, CO, USA). Slides were analysed using a well-established semi-quantitative scoring method (0-4) and scored separately for perivascular/vascular, lining layer and sub-lining layer regions [21].

Ex vivo synovial explants
RA synovial biopsies taken at the time of arthroscopies were sectioned into two equal pieces and cultured in 96well plates in full RPMI medium. This culture models maintains the synovial architecture and cell-cell contact, and therefore more closely reflects the in vivo environment. For stimulation experiments, RA synovial biopsies were cultured with Pam3CSK4 (1 μg/ml) or basal control media for 24 h. For blockade experiments, biopsies were cultured in the presence of OPN301 (mouse IgG1 monoclonal anti-TLR2 antibody, a kind gift from Opsona Therapeutics, Dublin, Ireland, 1 μg/ml) or IgG isotype control (mouse IgG1 isotype control, Opsona Therapeutics; 1 μg/ml). For each experiment, three technical replicates are combined (three individual biopsies from one patient) to give one biological sample, and this was performed on a number of different patients. This is important to allow for heterogeneity within the patient joint and between patients. Following incubation, culture supernatants were harvested and assayed for MMPs by enzyme-linked immunosorbent assay (ELISA) and zymography. For further functional experiments, 10 % conditioned media from OPN301 or IgG1-treated ex vivo synovial tissue was co-cultured with primary RASFC and invasion, migration and MMP expression was assessed as outlined below.

Culture of HMVEC
Human microvascular endothelial cells (HMVEC) (Lonza, Walkersville, MD, USA) were incubated in endothelial cell basal medium (EBM: Lonza) supplemented with endothelial cell growth medium (EGM) microvascular bullet kit containing 25 ml FCS, 0.5 ml hEGF, 0.5 ml hydrocortisone, 0.5 ml gentamicin, 0.5 ml bovine brain extract (Lonza). Cells were grown to confluence in T75cm flasks at 37°C in humidified air with 5 % CO 2 prior to being harvested with trypsin-EDTA (Lonza). HMVEC between the fourth and eight passages were used for experiments.

Ex vivo RA fibroblast outgrowths
To examine RA synovial outgrowths, an ex vivo synovial tissue explant model was utilised [22]. A total of 50 μl of matrigel (Bio-sciences) was added to each well of a 96well plate and incubated for 1 h at 37°C. Following this, RA synovial tissue was sectioned and placed in matrigel wells with RPMI 1640 medium supplemented with 10 % FCS (Gibco-BRL), 10 ml of 1 mmol/l HEPES (Gibco-BRL), penicillin (100 units/ml; Bio-sciences), streptomycin (100 units/ml; Bio-sciences) and fungizone (0.25 μg/ml; Bio-sciences). RA explants were stimulated with TLR2 ligand Pam3CSK4 (1 μg/ml) over a time course of 1-15days. Supernatants were collected every 4 days and replenished with fresh media and experimental agents. Images were taken using a phase-contrast microscope (a Nikon TMS microscope (Nikon Corp., Tokyo, Japan) linked to a Canon S70 camera (Canon Inc., Tokyo, Japan)).

Immunofluorescent staining for F-actin
Primary RASFC were seeded sparsely into 8-well chamber slides and serum starved for 24 h and subsequently cultured with Pam3CSK4 (1 μg/ml) for a further 24 h. Slides were washed with PBS and fixed with 1 % paraformaldehyde in PBS for 20 min. To visualise F-actin, slides were stained with Oregon Green® phalloidin (Molecular Probes, Leiden, The Netherlands). Nuclei were counterstained with DAPI (Sigma-Aldrich, St. Louis, MO, USA). Stained cells were visualised with a Leitz DM40 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured using the AxioCam system and AxioVision 3.0.6. software (Carl Zeiss Inc., Thornwood, NY, USA).
β integrin-mediated cell adhesion array The Chemicon Integrin-Mediated Cell Adhesion Array Kit (Chemicon International Inc., Temicula, CA, USA) utilises monoclonal antibodies generated against human integrins, which are immobilised onto a goat anti-mouse antibody-coated microplate and used to capture cells expressing these integrins on their cell surface. RASFC were grown to confluence, serum starved overnight and then cultured with Pam3CSK4 (1 μg/ml) for an additional 24 h. Single-cell suspensions were prepared using a non-enzymatic dissociation buffer (Sigma-Aldrich). A total of 100 μl (1 × 10 5 cells in adhesion buffer) was added to each well of the Beta Integrin-Mediated Cell Adhesion Array Kit (Chemicon). After incubation for 2 h at 37°C/5 % CO 2 , wells were washed and stained, and cell-bound stain was solubilised in extraction buffer. Integrin expression was determined by measuring the absorbance at 570 nm.

Wound repair assays
RASFC and HMVEC were seeded onto 48-well plates and grown to confluence. A single scratch wound was induced through the middle of each well with a sterile pipette tip. Cells were subsequently stimulated for 24 h with Pam3CSK (1 μg/ml). RASFC and HMVEC migration across the wound margins from 12 to 24 h was assessed and photographed using a phase-contrast microscope (Nikon TMS microscope linked to a Canon S70 camera). To assess if the β1-integrin signalling mediates Pam3CSK4-induced cell migration, experiments were also performed in the presence or absence of anti-β1-integrin (10 μg/ml) or isotype-matched non-immune IgG (10 μg/ml). Semi-quantitative analysis of cell repopulation of the wound was assessed by two blinded observers as previously described [23]. Briefly, images of the scratch wound assays were taken at x20 magnification. The mean closure of the wound was manually calculated from the average of three individual measurements from each wound. This process was repeated for all technical replicates. Measurement of scratches at time 0 were designated as 100 % open. From this, the percentage of closure for all scratches was calculated. Twenty-four percent wound closure represents the distance the cells migrated into the scratch wound. Data is represented as mean ± SEM. Furthermore to assess the effect of blocking TLR2 on cell migration, RASFC were incubated with 10 % conditioned media from ex vivo RA synovial explants cultured either with OPN301 (1 μg/ml) or IgG isotype control (1 μg/ml) for 24 h.

Transwell migration assay
Biocoat Matrigel™ Invasion Chambers (Becton Dickinson, Oxford, UK) were used to assess RASFC and HMVEC invasion. Cells were seeded at a density of either 2.5 × 10 4 cells per well in the migration chamber on 8 μm membranes pre-coated with matrigel. Cells were incubated for either 24 h (HMVEC) or 48 h (RASFC). Initial experiments assessed the effect of Pam3CSK4 (1 μg/ml) on HMVEC and RASFC invasion. To assess if the β1-integrin signalling pathways mediate Pam3CSK4-induced cell invasion, experiments were also performed in the presence or absence of anti-β1-integrin (10 μg/ml) or isotype-matched nonimmune IgG (10 μg/ml). To determine if TLR2 blockade could further inhibit invasion, RASFC were cultured in the presence of 10 % RA ex vivo explant-conditioned media previously cultured with either anti-TLR2 antibody OPN301(1 μg/ml) or IgG control (1 μg/ml). Non-migrating RASFC and HMVEC were removed from the upper surface by gentle scrubbing. Migrating cells attached to the lower membrane were fixed with 1 % glutaraldehyde and stained with 0.1 % crystal violet. Cells from five random highpower fields for each well were counted to assess the average number of migrating cells [24].

Rac1 pull-down assay
Rac1 activity was determined using an Active Rac1 Pull-Down Detection Kit (Thermo Scientific, Pierce, Rockford, IL, USA) as per the manufacturer's protocol. RASFC were stimulated with Pam3CSK4 (1 μg/ml) for 12 h. After treatment, cells were rinsed once with ice-cold PBS and scraped into 500 μl of lysis buffer. Samples were vortexed and centrifuged at 16,000 g for 15 min at 4°C and the supernatant was transferred to a new tube. Active Rac1 was then affinity purified with GST-Pak-PBD (Pak 1-p21-binding domain), separated by SDS-PAGE and measured by Western blotting using anti-Rac1 antibodies and total Rac1 protein. To assess if the β1-integrin signalling pathways mediate Pam3CSK4induced Rac1 activation, experiments were also performed in the presence or absence of anti-β1-integrin (10 μg/ml) or isotype-matched non-immune IgG (10 μg/ml). The signal intensity of the appropriate bands on the autoradiogram was calculated using the Kodak EDAS 120 System (Kodak, Rochester, NY, USA). Densitometry analysis performed out using ImageJ software. Data is expressed as fold change compared to β-actin control.

Matrix metalloproteinase expression
RASFC were seeded in 48-well plates containing RPMI plus supplements for 24 h. Following 24 h of incubation in serum-free RPMI 1640, cells were stimulated with Pam3CSK4 (1 μg/ml), experiments were also performed in the presence or absence of anti-β1-integrin (10 μg/ml) or isotype-matched non-immune IgG (10 μg/ml). For inhibitory experiments, RA ex vivo biopsies were treated as outlined above. Supernatants were harvested and levels of MMP-3, MMP-1 and tissue inhibitor of matrix metalloproteinase (TIMP)-3 were measured by specific ELISA (R&D Systems) according to the manufacturer's conditions. The ELISA standard ranges were 10 ng/ml to 0.156 ng/ml for MMP-3 and MMP-1 and 4,000 pg/ml to 62.5 pg/ml for TIMP-3.

mRNA extraction and PCR analysis
RASFC were grown to confluence, cultured in serumfree RPMI medium for 24 h and then stimulated with Pam3CSK4 (1 μg/ml) for a further 24 h. Experiments were also performed in the presence or absence of anti-β1-integrin (10 μg/ml) or isotype-matched non-immune IgG (10 μg/ml). Total RNA was isolated using RNeasy Mini Kit (Qiagen, Venlo, The Netherlands) according to the manufacturer's specifications. Purity of RNA was measured and samples with a ratio over 1.8 (260:280 nm) were used in subsequent experiments. Total RNA (200 ng) was reverse transcribed to cDNA. Relative quantification of gene expression was analysed with pre-optimised conditions using Lightcycler-480 PCR technology (Roche Diagnostics, Lewes, UK). Specific primers for β1-integrin (Hs00559595_m1) were used and primers for 18S (Hs99999901_sl) ribosomal RNA were used as an endogenous control (Applied Biosystems, Carlsbad, CA, USA).
Statistical analysis SPSS 15.0 system (SPSS Inc, Chicago, IL, USA) for Windows was used for statistical analysis. Wilcoxon signedrank test or Mann-Whitney was used for analysis of non-parametric data. Student t test was used for parametric data. p values of less than 0.05 ( * p <0.05) were determined as statistically significant. All data is represented as mean ± SEM.

TLR2 induces cell migration in RASFC and HMVEC
RASFC and HMVEC monolayers were wounded and stimulated in the presence or absence of Pam3CSK4 (1 μg/ml). Figure 1i shows representative images of wound repair in response to Pam3CSK4 for RASFC and HMVEC, where repopulation of wound margins was observed. Semiquantitative analysis demonstrated a significant increase in cell migration in response to Pam3CSK4 (Fig. 1ii).

Pam3CSK4 induces ex vivo fibroblast outgrowth and cytoskeletal rearrangement
To further probe the effects of TLR2 activation on cell migration/invasion the effect of Pam3CSK4 on cytoskeletal dynamics was examined using F-actin staining. Figure 3a, in panels (i-iii), under basal or resting conditions, intact bundles of stress fibres are clearly visible and are orientated along the cells in a uni-directional manner. This is indicated in Fig. 3a (i) and (ii) by red arrows. Figure 3a panels (iv-vi) demonstrate the effects of Pam3CSK4 on the F-actin cytoskeletal architecture and cell shape. Pam3CSK4 has a dramatic effect on the architecture of the cell. It induces thin actin-rich extensions of the cytoplasm and membrane ruffling (observed in Fig. 3a (iv)), indicated by white arrows). It also induces filopodial and lamellipodiallike structures (observed in Fig. 3a (v)), indicated by white arrows). Lower magnification images (x40) depicted in Fig. 3a capture cells either under basal (iii) or Pam3CSK4induced (vi) conditions, where these key cellular changes Fig. 1 Representative photomicrograph demonstrating cells repopulating the wound in response to Pam3CSK4 (1 μg/ml) in RASFC and HMVEC (i). Bar graph quantifying RASFC and HMVEC (both n = 4) 24 h migration in response to Pam3CSK4 (ii). Data is represented as mean ± SEM, * p <0.05, significantly different to control. HMVEC human microvascular endothelial cells, RASFC rheumatoid arthritis synovial fibroblast cells are uniform and highly noticeable amongst groups of cells. To further assess the role of TLR2, we cultured ex vivo RA whole tissue synovial explants embedded in matrigel for 15 days with Pam3CSK4 (1 μg/ml). Images of RA synovial outgrowths are shown in Fig. 3b where a significant increase in synovial outgrowth is demonstrated in response to Pam3CSK4 compared to basal control (indicated by black arrows) where minimal outgrowth was observed. Pam3CSK4 significantly increased expression of β1-integrin on the cell surface of RASFC (p <0.05) (Fig. 3c). No significant differences were observed in the levels of β2, β3, β4, β6, αVβ5 or α5β1 integrins. Figure 3d demonstrates a 2.6-± 0.66 fold increase in β1-integrin mRNA following Pam3CSK4 stimulation. Furthermore Pam3CSK4 induced active Rac1 compared to basal control in RASFC, without change in global Rac1 expression (Fig. 3e). Figure 3f shows

Pam3CSK4-induced cell migration and invasion is mediated through β1-integrin
To examine if Pam3CSK4-induced migration and invasion processes are in part mediated by the β1integrin signalling pathways, RASFC and HMVEC were stimulated with Pam3CSK4 in the presence or absence of neutralizing antibody against β1-integrin or an isotype-matched anti-IgG control. Figure 5a demonstrates Pam3CSK4-induced RASFC and HMVEC migration was inhibited in the presence of anti-β1integrin. Representative images of RASFC are shown in Fig. 5a (i). Semi-quantitative analysis demonstrated a significant decrease in Pam3CSK4-induced cell migration in response to anti-β1-integrin in both RASFC (1 μg/ml) (n = 5). e Representative Western blot for activated Rac1, total Rac1 and β-actin control in RASFC following 24 h stimulation with Pam3CSK4 (1 μg/ml). f Densitometry quantification of active Rac1 in RASFC (n = 3). Data is represented as mean ± SEM, * p <0.05, significantly different to control. RA rheumatoid arthritis, RASFC rheumatoid arthritis synovial fibroblast cells (Fig. 5a (ii)) and HMVEC (Fig. 5a (iii)) (p <0.05). Pam3CSK4-induced RASFC invasion was inhibited in the presence of anti-β1-integrin (p <0.05), with no effect observed for the IgG control ( Fig. 5b (i, ii)). Similar effects were observed for HMVEC ( Figure S1A in Additional file 1). Furthermore, Pam3CSK-induced RASFC Rac1 activation was inhibited by anti-β1integrin in RASFC, with no change in global Rac1 expression ( Fig. 5c; Figure S1B in Additional file 1). Interestingly, anti-β1-integrin had no effect on Pam3CSK4-induced MMP-3 (p = 0.87) (data not shown), suggesting that these were independent of β1integrin.

OPN301 inhibits migration and invasion RA explants and RASFC
To further examine the therapeutic potential of blocking these pathways we assessed the effect of an anti-TLR2 antibody OPN301 in the ex vivo RA synovial explant model. OPN301 significantly reduced spontaneous secretion of MMP-3 from the RA explants compared to IgG control (p <0.05) (Fig. 6a (i)), resulting in a significant reduction in the MMP-3:TIMP-3 ratio (Fig. 6a (iii)) (p <0.05), thus favouring an MMP/TIMP balance against joint destruction. This was also reflected in the expression of MMP-2 and MMP-9 both of which were inhibited in the presence of OPN301 (Fig. 6b, lane 2) compared to IgG control (Fig. 6b, lane one). OPN301 reduced MMP-1 secretion from explants but this did not reach significance ( Figure S1C in Additional file 1). RASFC wounded monolayers were cultured with conditioned media from RA synovial explants treated with OPN301 or IgG control. OPN301-conditioned media inhibited RASFC cell migration across the wound, in contrast to those cultured with IgG control-conditioned media, which had no inhibitory effect (Fig. 6c). Finally, Fig. 6d demonstrates OPN301-conditioned media also inhibited RASFC invasion through a matrigel compared to IgG control. Figure 6d (ii) shows a representative bar graph demonstrating a decreased number of invading RASFC following incubation with OPN301-conditioned media.

Discussion
RA is a chronic inflammatory disease characterised by increased angiogenesis, cell migration and synovial hyperplasia. TLR2 has been implicated in the pathogenesis of RA, however, the functional mechanisms and signalling pathways mediating its effect on synovial migration and invasion have not been extensively investigated. In this study, we demonstrate that TLR2 activation induces RASFC and HMVEC cell migration, invasion, RA synovial explant outgrowths, in addition to an increase in MMP production. Furthermore, we show that TLR2-induced migration/invasion is partly mediated by β1-integrin and downstream alterations in cytoskeletal dynamics and Rac1 activation. Finally, using an ex vivo RA synovial explant model and RASFC, we demonstrated that blockade of TLR2 significantly inhibited MMP secretion, RASFC migration and invasion.
The induction of MMP-3 by TLR2 in RASFC is consistent with a study by Yokota et al. showing TLR2 can synergise with NOD1 in the induction of MMP-3 in RASFC [9]. In addition, a recent study demonstrated that microRNA-19a/b can regulate MMP-3 secretion through activation of TLR2 [10]. Previous studies have shown that MMP-1 and MMP-3 correlate with erosive progression in RA [32]. In addition, studies have shown an association between MMP-3, cartilage neoepitopes, and serum amyloid A (SAA) [33] and demonstrated that MMP-3 levels at baseline predict radiographic progression following TNF-α inhibitor (TNFi) therapy [32]. Furthermore, in human periodontal fibroblasts, TLR2 activation can induce MMP-1 and MMP-3 via p38, JNK and NF-ĸB [34].
We also demonstrated a significant shift in both the MMP-3/TIMP-3 and MMP-1/TIMP-3 ratios suggesting TLR2 activation in the RA joint favours destructive pathways. TLR2 activation inhibited TIMP-3 in RA synovial explants but not RASFC. The expression of TIMP-3 in RA synovial tissue lysates has been previously reported, however, studies did not identify the cellular source of TIMP-3 and the majority of studies in RA only focus on the expression of TIMP-3 in RASFC. However, studies in different disease states and mouse models have demonstrated expression of TIMP-3 in endothelial cells [35], chondrocytes [36] and macrophages [37]. As endothelial cells and macrophages are two prominent cell types in the RA synovium, this suggests that they may be a potential source of the TIMP-3 secreted from explants in response to TLR2 activation.
To further probe the precise mechanisms involved in mediating TLR2-induced synovial invasion, we investigated the role of the β1-integrin signalling pathway. We Fig. 6 a Quantification of RA synovial tissue spontaneous secretion of MMP-3 (i), TIMP-3 (ii) and the MMP-3/TIMP-3 ratio (iii) following culture with OPN301 (1 μg/ml) or IgG control (1 μg/ml) (n = 4). b Decreased MMP-2 and MMP-9 expression in response to culture with OPN301 (1 μg/ml) compared to anti-IgG control antibody (1 μg/ml) in RA synovial culture supernatants as assessed by gelatin zymography (n = 3) (c). Representative photomicrograph demonstrating inhibition of RASFC migration in response to conditioned media from ex vivo RA synovial tissue treated with OPN301 (1 μg/ml) compared to IgG control (1 μg/ml) (n = 3) (d). Representative photomicrographs showing OPN301-conditioned media (1 μg/ml) inhibits RASFC invasion compared to IgG-conditioned media (i). Bar graph quantifying RASFC invasion (ii) (n = 4). Data is represented as mean ± SEM, * p <0.05, significantly different from control. IgG immunoglobulin G, MMP matrix metalloproteinase, RA rheumatoid arthritis, RASFC rheumatoid arthritis synovial fibroblast cells, TIMP tissue inhibitor of metalloproteinase demonstrated that TLR2 activation preferentially induced β1-integrin binding with no effect observed for β2, β3, β4, β6, αVβ5 or α5β1. Further downstream, we showed that Pam3CSK4-induced Rac1 activation, which is critical for cell actin assembly at the cell periphery promoting cell movement. The effect of TLR2 on β1-integrin and Rac1 was parallelled by alteration in cytoskeletal dynamics where a dramatic effect on the cytoskeletal integrity was observed in both RASFC and HMVEC, consistent with the localised expression of β1-integrin in RA synovial tissue. Finally, we demonstrated that β1-integrin mediated TLR2 function in RASFC and HMVEC, by showing that TLR2-induced migrational and invasive processes were inhibited by neutralising antibodies against β1-integrin.
This is the first study to demonstrate that the β1-integrin pathways specifically mediates TLR2-induced invasion in the inflamed joint, with minimal effect observed for other β-integrins. This is consistent with previous studies showing β-integrin expression on RASFC is associated with their enhanced binding to extracellular matrix (ECM) [38]. The β1-integrin receptor plays a key role in the attachment of RASFC to ECM components and the subsequent invasion of cartilage [18,38] and can induce Rho GTPases, specifically Rac1 in chondrocytes and murine macrophages [39,40]. Integrin receptors, p-focal adhesion kinase (FAK) and p-paxillin are significantly increased in RA synovial tissue [41]. β1-integrin is known to be involved in angiogenesis and in the response of T cells to fibronectin-induced chemotactic signals [39,42]. While little is known about the cross-talk between TLR2 and integrin-FAK-mediated pathways in the RA synovium, previous studies have reported that TLR2 mediates macrophage cytoskeletal rearrangement, promoting macrophage spreading and polarisation, a process required during extravasation and migration [43]. Furthermore, studies have also reported that TLR2 activation induces angiogenesis and EC migration in response to oxidative stress via Rac1 [44,45] and TLR2(−/−) and MyD88(−/−) macrophages have impaired Rac1 and PI3K function [46].
The mechanisms by which TLR2 mediates β1-integrin action remains unclear. β1-integrin is a transmembrane receptor that is a bridge for cell-cell and cell-ECM interactions, which in turn activates the cytoskeletal dynamics. Ligands for β1-integrin include fibronectin, vitronectin, collagen, and laminin. While no studies in RA cells have shown a direct link between TLR2 and these ligands, a few studies in other disease cells have shown that TLR2 and 4 can regulate the expression of different ECM proteins. Vitronectin and fibronectin have been shown to be essential for TLR2-mediated processes in vitro, both of which are known to be induced in RA [47][48][49][50][51]. Furthermore, while no studies have yet shown a direct link between TLR2 and β1-integrin, we and others have shown that TLR2 is a functional receptor for acute-phase (A)-SAA, which is known to induce cell migration through integrincytoskeletal-mediated pathways in the joint [33,52]. Previous studies have also shown interactions between A-SAA and ECM glycosaminoglycans, and with laminin and vitronectin-induced adhesion and migration in a β1-integrin-dependent manner [53,54].
In this study, β1-integrin blockade had no effect on MMP-3 expression in RASFC. β1-integrin blockade is required to block MMP-2 activation induced by collagen I (Col I) [55]. Other studies have demonstrated in mice lacking α2β1 integrin, inhibition of MMP-3 expression in RASFC and cartilage degradation [18]. MMP can in turn regulate integrins, with studies showing the regulatory action of MMP-13 on α11β1-dependent matrix remodelling in fibroblasts [56]. However, a study by Ozeki et al., demonstrated complimentary but independent action of α2 integrin and MMP-3-mediated signalling cascades in driving mouse embryonic stem cell differentiation into odontoblast-like cells [57]. Interactions between integrins and MMP in the regulation of matrix remodelling thus may be complimentary and bi-directional with differential sensitivity and activation mechanisms in different cell types or tissues [58].
Finally, we used an ex vivo RA synovial tissue (ST) explant model to investigate the role of TLR2 blockade on RA synovial invasion. This model maintains the cell-cell contact of the complex mix of different cell types whose interaction contributes to the pro-inflammatory environment in the RA joint. RA synovial explants spontaneously release key pro-inflammatory mediators and therefore are ideal for examining potential therapeutic molecules. We demonstrated that OPN301 inhibited spontaneous secretion of MMP-3, MMP-2, and MMP-9 and inhibited the MMP3/TIMP3 ratio in favour of decreased cartilage degradation. We also demonstrated that OPN301-conditioned media inhibited RASFC migration and invasion, suggesting OPN301 inhibited spontaneous release of soluble mediators that are involved in migratory and invasive mechanisms in the RA joint. When RASFC were cultured with OPN301 and IgG alone no inhibitory effect on RASFC migration in response to OPN301 was observed ( Figure S1D in Additional file 1). This further suggests endogenous TLR2 ligands are present in the inflamed joint. However, as OPN301 had no direct effect on RASFC migration, ligands may be secreted by other cells in RA synovial tissue. While no ligand has been defined, the existence of a ligand is supported by studies which showed that macrophages were activated in a MyD88 and Mal-dependent manner when cultured with conditioned media from RA synovial explants [8]. Several potential ligands have been suggested such as fibronectin fragments, heat shock protein 70, hyaluronidase oligosaccharides, high mobility group protein 1 (HMBG-1) and glycoprotein 96 (GP96) all of which have been identified in the RA joint [59][60][61][62][63]. These studies support our data suggesting the presence of TLR2 ligands actively secreted from RA ex vivo synovial tissue.

Conclusions
In conclusion, we demonstrate that activation of TLR2 induces RASFC migration, invasion and extracellular matrix breakdown, through induction of MMPs. We show that TLR2-induced migration/invasion is partly mediated by β1-integrin and downstream alterations in cytoskeletal dynamics. Finally, using an ex vivo RA synovial explant model, we demonstrate that TLR2 blockade significantly inhibits MMP expression and migration, suggesting the presence of an endogenous ligand. This study further supports that hypothesis that TLR2-induced signalling pathways are involved in the pathogenesis of RA.