Alteration of matrix metalloproteinase-3 O-glycan structure as a biomarker for disease activity of rheumatoid arthritis

Background Nearly all secreted proteins are glycosylated, and serum glycoproteins that exhibit disease-associated glycosylation changes have potential to be biomarkers. In rheumatoid arthritis (RA), C-reactive protein (CRP), and matrix metalloproteinase-3 (MMP-3) are widely used as serologic biomarkers, but they lack sufficient specificity or precision. We performed comparative glycosylation profiling of MMP-3 using a recently developed antibody-overlay lectin microarray technology that allows semicomprehensive and quantitative analysis of specific protein glycosylation to develop an RA-specific disease activity biomarker. Methods Serum was taken from patients with RA (n = 24) whose disease activity was scored using composite measures, and MMP-3 was immunoprecipitated and subjected to lectin microarray analysis. A disease activity index (DAI) based on lectin signal was developed and validated using another cohort (n = 60). Synovial fluid MMP-3 in patients with RA and patients with osteoarthritis (OA) was also analyzed. Results Intense signals were observed on a sialic acid-binding lectin (Agrocybe cylindracea galectin [ACG]) and O-glycan-binding lectins (Jacalin, Agaricus bisporus agglutinin [ABA], and Amaranthus caudatus agglutinin [ACA]) by applying subnanogram levels of serum MMP-3. ACG, ABA, and ACA revealed differences in MMP-3 quantity, and Jacalin revealed differences in MMP-3 quality. The resultant index, ACG/Jacalin, correlated well with disease activity. Further validation using another cohort confirmed that this index correlated well with several DAIs and their components, and reflected DAI changes following RA treatment, with correlations greater than those for MMP-3 and CRP. Furthermore, MMP-3, which generated a high ACG/Jacalin score, accumulated in synovial fluid of patients with RA but not in that of patients with OA. Sialidase digestion revealed that the difference in quality was derived from O-glycan α-2,6-sialylation. Conclusions This is the first report of a glycoprotein biomarker using glycan change at a local lesion to assess disease activity in autoimmune diseases. Differences in the degree of serum MMP-3 α-2,6-sialylation may be a useful index for estimating disease activity.


Background
Rheumatoid arthritis (RA) is a common autoimmune disease characterized by synovial inflammation and hyperplasia. The hyperplastic synovium produces vast amounts of matrix metalloproteinases (MMPs) that degrade cartilage matrix as well as synovial inflammatory cytokines such as macrophage colony-stimulating factor and receptor activator of nuclear factor κB ligand that promote osteoclastogenesis and disrupt joint structure [1].
To prevent RA progression, it is important to define the treatment target, such as remission or at least low disease activity; to assess disease activity using composite measures; and to adapt therapy if the target is not achieved within a particular time frame. This is referred to as a treat-to-target strategy [2,3]. The Disease Activity Score in 28 joints (DAS28), which combines evaluation by a rheumatologist, laboratory test results, and the patient global assessment, has commonly been used to assess disease activity [4,5]. Recently, new indices such as the Simplified Disease Activity Index [6] and Clinical Disease Activity Index [7], which simplified the DAS28, have been developed.
C-reactive protein (CRP) and MMP-3 are widely measured as serum markers. Although CRP, an acute phase protein, reacts to joint inflammation, it cannot distinguish RA activity and other inflammatory conditions such as infectious disease. In contrast, MMP-3 is characterized as a more specific indicator of synovial inflammation. It was originally identified as a protein secreted from RA synovial fibroblasts [8]. MMP-3 degrades various extracellular substrates, including proteoglycan, fibronectin, laminin, and type 4 collagen, in addition to activating pro-MMPs. Thus, MMP-3 is thought to contribute to cartilage destruction in RA pathophysiology [9]. Serum MMP-3 is elevated in diseases that involve joint synovitis, including RA, reactive arthritis, psoriatic arthritis, and crystal arthritis, but not in osteoarthritis (OA) or systemic inflammatory conditions such as sepsis [10,11]. However, correlation with disease activity indices (DAIs) is superior in acute phase proteins compared with serum MMP-3 [12,13]. Thus, development of an RA-specific disease activity biomarker is needed.
It is known that almost all secreted proteins are glycosylated, that glycosylation patterns are influenced by cellular differentiation, and that serum glycoproteins exhibiting disease-associated glycosylation changes have potential to be biomarkers [14]. For example, serum α-fetoprotein (AFP), a commonly used hepatocellular carcinoma biomarker, can be fractionated into three glycosylation patterns-L1, L2, and L3-using Lens culinaris agglutinin lectin. Because AFP-L3 is produced only by hepatocellular carcinoma, measurement of AFP-L3 rather than total AFP provides superior sensitivity and specificity [15,16].
Although analysis of carbohydrate chains has been difficult because of their repetitive sequence and structural variety, the recently developed antibody-overlay lectin microarray technology allows semicomprehensive and quantitative analysis of protein glycosylation patterns [14]. Kuno et al. [17] showed that the glycosylation pattern of serum Mac-2-binding protein, which had previously been reported as a quantitative marker for tumor progression and metastasis [18], gradually changes during liver fibrosis progression and thus serves as a biomarker for liver fibrosis.
In the present study, we focused on an existing biomarker, MMP-3, and examined the association between its glycosylation pattern and RA disease activity. We report on a new, sensitive biomarker that is based on local inflammation and can be assessed using protein glycosylation changes.

Patients and samples
RA serum and synovial fluid samples were collected at Keio University Hospital. All patients fulfilled the 2010 American College of Rheumatology/European League Against Rheumatism classification criteria for RA [19]. Written informed consent was obtained from all individuals. This study was approved by the institutional review board of Keio University School of Medicine and the National Institute of Advanced Industrial Science and Technology, and it was conducted in compliance with the Declaration of Helsinki.

Sample preparation
Serum MMP-3 enrichment was performed as previously described [14]. Serum or synovial fluid samples were precleared with 100-μg streptavidin beads (FG beads; Tamagawa Seiki, Iida, Japan) at 4°C for 30 minutes in Tris-buffered saline containing 1 % Triton X-100 (TBS-Tx). Protein G-purified biotinylated anti-MMP-3 antibody (200 ng; R&D Systems, Minneapolis, MN, USA) was added to precleared samples and incubated overnight at 4°C. Antibody complexes were incubated with 100-μg streptavidin beads at 4°C for 30 minutes, washed three times with TBS-Tx. MMP-3 and biotinylated anti-MMP-3 antibody were eluted by boiling at 95°C for 5 minutes in Tris-buffered saline containing 0.2 % sodium dodecyl sulfate. Antibodies were removed by incubation with 100-μg streptavidin beads at 4°C for 30 minutes. Supernatants were collected and used for the next assay as immunoprecipitation (IP) samples. For sialic acid digestion, IP samples were incubated with Glyko Sialidase A (PROzyme, Hayward, CA, USA) at 37°C for 1 h before applying them to lectin microarrays (LecChip; GlycoTechnica, Yokohama, Japan).

Antibody-overlay lectin microarray
Antibody-overlay lectin microarray assays were performed as described previously [20,21]. Briefly, IP samples were diluted to 60 μl with PBS containing 1 % Triton X-100 (PBS-Tx) and applied to lectin microarrays. After incubation overnight at 20°C, human serum immunoglobulin G (IgG) (2 μl, approximately 10 mg/ml; Sigma-Aldrich, St. Louis, MO, USA) was added to the array, followed by 30 minutes of incubation. The reaction solution was discarded, and the array was washed with PBS-Tx. Biotinylated anti-MMP-3 antibody (100 ng) was applied to the array, incubated at 20°C for 1 h, washed with PBS-Tx, and incubated at 20°C for 30 minutes with 200 ng of cyanine 3-labeled streptavidin (GE Healthcare, Little Chalfont, UK). The array was rinsed with PBS-Tx and scanned using an evanescent-field fluorescence scanner (GlycoStation; GlycoTechnica). All data were analyzed with ToolsPro Suite software V1.5 (GlycoTechnica). Net intensity was calculated by subtracting the mean background from the mean signal intensity of three spots per lectin. A schematic overview of this assay is shown in Fig. 1.

Serum and synovial MMP-3 measurement
Serum MMP-3 was measured using a latex turbidimetric immunoassay (Eiken Chemical, Tokyo, Japan). Synovial fluid MMP-3 was measured by enzyme-linked immunosorbent assay (R&D Systems) according to the manufacturer's instructions.

Statistics
Correlation analysis was conducted using the Pearson product-moment correlation coefficient (JMP 11 software; SAS Institute, Cary, NC, USA). We considered p < 0.05 to be significant.

MMP-3 has an O-glycan cluster
MMP-3 is a serum protein present in relatively low abundance (10-100 ng/ml in healthy people and 50-1000 ng/ml in patients with RA) [11]. Therefore, MMP-3 was enriched from precleared serum (40 μl) of 24 patients with RA by IP (Table 1). To reduce contaminant, we carefully selected streptavidin beads [22] and used anti-MMP-3 antibody after protein G purification, and removed the antibody from IP eluate. Although small amounts of contaminated protein remained in IP samples, we confirmed that the contaminant did not inhibit lectin signals by spike testing using recombinant MMP-3. The yield and stability of IP samples was checked by Western blot analysis (Fig. 2a), and we confirmed that there were no extra bands in all samples. Because we used the same antibody for Western blot analysis and antibody-overlay lectin microarray experiments, almost all lectin signals were thought to be derived from MMP-3. Aliquots of the IP samples, including approximately 1 ng of MMP-3, were subjected to antibody-overlay lectin microarray.
Representative results from two patients, one with high disease activity and the other in remission, are shown in Fig. 2b. Although trace amounts of MMP-3 were applied, four lectins showed significant signal in all samples: Agrocybe cylindracea galectin (ACG), Agaricus bisporus agglutinin (ABA), Amaranthus caudatus agglutinin (ACA), Fig. 1 Schematic overview of the antibody-overlay lectin microarray. A total of 45 kinds of lectins are spotted in triplicate on the array slide. Immunoprecipitation samples were applied to them, and glycoproteins bound to the lectins through their glycans. After washing, the lectins that bound to matrix metalloproteinase (MMP)-3 glycans were detected using anti-MMP-3-specific antibody and Jacalin. ACG recognizes α-2,3 sialic acid [23], ABA, ACA, and Jacalin commonly recognize the T antigen (core 1 structure) of O-glycan [21]. Such a unique binding pattern at a low concentration has been observed for mucin-like O-glycosylated proteins such as mucin 1 [24], podoplanin [21], and immunoglobulin A (IgA) [20]. The estimated glycan structure of the MMP-3 molecule was discussed later in this article. We did not observe a signal for N-glycan binding, although MMP-3 has a potential Nglycosylation site. It is well known that interaction of a glycoprotein that has a single N-glycan, such as AFP, with lectins is weak and difficult to detect at low analyte concentrations. To our knowledge, this is the first report to describe MMP-3 as having an O-glycan cluster.

ACG/Jacalin ratio reflects RA activity
We observed differences between lectin signals among patients with RA, prompting us to next evaluate the  Therefore, we evaluated the correlation between representative lectin signals and MMP-3 quantity (Fig. 2c). The results show that ACG, ABA, and ACA signals correlated well with MMP-3, regardless of RA disease activity. This suggests little variation in the structure of ACG, ABA, and ACA binding glycans. Jacalin signal, however, correlated poorly with MMP-3, suggesting that there were variations in the structure of Jacalin-binding glycans. Especially among patients with a high RA disease activity, Jacalin signals seemed to be relatively low compared with quantity of MMP-3; therefore, we thought it might be possible to use Jacalin as the RA activity biomarker. However, there are two problems with using Jacalin signals as a disease activity marker: (1) lectin signals is influenced by serum MMP-3 concentration; and (2) because the relationship between disease activity and Jacalin signals was negatively correlated, use of Jacalin signals is impractical. To overcome these problems, we took the ratio of the Jacalin and ACG signals, which had the strongest correlation to MMP-3 quantity. As expected, the ACG/ Jacalin index was positively correlated with RA disease activity (Fig. 2d). This result suggests that profiling MMP-3 glycosylation patterns produced in local RA lesions may serve as a disease activity marker.

ACG/Jacalin index as an RA disease activity marker
To confirm the association between the ACG/Jacalin index and disease activity, we recruited a second patient cohort and measured the ACG/Jacalin index in the same manner. This validation cohort included 60 serum samples from 30 patients with RA before and after treatment. The median treatment period was 12 months (interquartile range 11-12 months) ( Table 2).
The correlation between composite measures and their components and ACG/Jacalin, MMP-3, and CRP are shown in Table 3. Consistent with previous reports [12,13], CRP correlated better with DAIs than serum MMP-3. However, focusing on the glycosylation pattern, the ACG/ Jacalin index correlated well with disease activity, similar to CRP. This result indicates that the ACG/Jacalin index may serve as a disease activity marker.
Importantly, the ACG/Jacalin index correlated with each component of the composite measures. In particular, the correlation coefficient was high for the joint score and Physician Global Assessment. The correlations with CRP and MMP-3 were not strong, suggesting they may reflect different aspects of disease activity. Because the standard serum MMP-3 value in males and females is different, MMP-3 values were calculated using only female data (Table 3). A gender gap was not observed in the relationship between the ACG/Jacalin index and disease activity (Fig. 3).
To examine whether the ACG/Jacalin index reflects a change in disease activity, we assessed the correlation between the change in the ACG/Jacalin index and DAIs before and after treatment (Table 4). Surprisingly, the ACG/Jacalin index reflected the degree of disease activity change better than MMP-3 and CRP. Similar to previous results, a change in the ACG/Jacalin index was correlated with a change in each component of the composite measures. These results strongly suggest that the ACG/Jacalin index may serve as a useful RA disease activity marker.

Local RA lesions produce MMP-3 with elevated ACG/Jacalin index
We found that serum MMP-3 glycosylation patterns reflected RA disease activity, and we further examined synovial fluid MMP-3 to confirm that this change was   Table 5. Synovial MMP-3 was 100-fold higher than serum MMP-3 and higher in RA versus OA, consistent with previous reports [9,11]. To focus on glycosylation differences, an IP and lectin microarray assay was performed after making MMP-3 concentrations equal by dilution. Synovial IP sample yields were confirmed by Western blot analysis (Fig. 4a), with representative lectin signals from three patients with RA and three patients with OA (Fig. 4b). RA synovial fluid MMP-3 had a high ACG signal and a low Jacalin signal compared with synovial fluid of patients with OA, the same as for high RA disease activity as indicated by serum MMP-3. This result suggests that the increase in the ACG/Jacalin index resulted in part from MMP-3 produced in inflammatory RA lesions.
Taken together, MMP-3 O-glycan is highly α-2,6sialylated in the RA joint lesion, and the ACG/Jacalin index, which reflects changes in sialylation, may serve as a new RA disease activity biomarker.

Discussion
Abnormal glycosylation has been studied extensively in RA. Studies have shown that RA exhibits an increase in agalactosyl IgG [25]; a decrease in the GalNAc content  of IgA [26]; and more recently, extensive glycosylation of the anticitrullinated protein antibodies-IgG variable domain [27]. Notably, most were surrogate markers that were not expressed in diseased synovial tissue. Our data point to a new RA activity marker using a change in glycosylation of a glycoprotein directly expressed in, and secreted from, synovial tissue. Although CRP and MMP-3 are widely used to monitor RA, they do not demonstrate sufficient specificity to determine disease activity. Thus, RA disease activity markers, such as the multibiomarker disease activity test [28,29] and the leucine-rich α2 glycoprotein [30], were developed. However, it has been difficult to find a protein that specifically reflects RA disease activity. In this study, we focused on the glycan content of an existing marker, MMP-3, to determine both specificity and sensitivity metrics.
As previously mentioned, glycosylation is influenced by the state of the cell [14]. MMP-3 production is increased in joint synovitis and is continually produced even in noninflamed joints. However, our results show that MMP-3 glycans change under inflammatory conditions and that the ACG/Jacalin index seemed to distinguish disease-specific MMP-3 from total MMP-3. This is one reason why our marker correlated well with disease activity. In addition, MMP-3 expression is relatively restricted within the joint and partly leaks into the serum [11]. Thus, it might be possible to directly determine inflammation at local lesions without an effect of any other influences. Interestingly, the ACG/Jacalin index had a higher correlation coefficient for joint parameters than CRP or MMP-3, suggesting that this index is a more specific arthritis marker.
The ACG/Jacalin index has advantages as a disease activity marker, but further study is required. The method used to obtain lectin signals is slightly complicated, and thus a simplified method is needed. It is better to clarify the glycan structure of MMP-3 and to detect MMP-3 with altered glycan directly. Additionally, sensitivity and stability should be improved for practical use because serum MMP-3 concentration is quite low in some patients after treatment. Although serum MMP-3 was reported to be a radiological predictor [31][32][33], we could not examine the association between ACG/Jacalin index and radiological progression, owing to the limited number of patients. In addition to RA, MMP-3 glycans may be altered in other disease conditions that increase serum MMP-3, such as psoriatic arthritis, reactive arthritis, steroid use, and polymyalgia rheumatica [10,31], requiring measurement of ACG/Jacalin under various situations.
To our knowledge, this study shows for the first time that serum glycoproteins exhibiting disease-associated glycosylation changes have potential as disease-specific biomarkers in autoimmune diseases. In patients with RA, differences in serum MMP-3 α-2,6-sialylation may be a

Conclusions
We have developed a new, sensitive RA-specific biomarker based on MMP-3 glycosylation changes. The ratio of two lectin signals, ACG and Jacalin, correlated well with several DAIs and their components and also reflected DAI changes following RA treatment. Further studies are required to develop the practical use of this biomarker.   6). b Representative lectin signals derived from synovial MMP-3 (mean ± SD). c The structure of T and sialyl-T antigen. Jacalin recognizes the former. d Jacalin and ACG signals compared before and after sialic acid digestion (mean ± SD). e The estimated basic structure of MMP-3 O-glycan. ABA Agaricus bisporus agglutinin, UDA Urtica dioica agglutinin, ACA Amaranthus caudatus agglutinin, WGA wheat germ agglutinin