Figure 3
From: The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin
Effect of point mutation Arg27a to serine in B3 variable light chainregion (VL) complementarity determining region 1. Comparison of cardiolipin binding of IgG in COS-7 cell supernatants containing wild-type heavy chains expressed with B3VL or B3VLa. Presented as concentration of IgG in the supernatant versus optical density (OD) at 405 nm in the anti-cardiolipin ELISA.