Table 3 Up- and down-regulated genes in osteoarthritis (OA) fibroblast-like synoviocytes (FLSs) stimulated with protein I/II. Confluent OA FLSs were stimulated with protein I/II for 4 hours or left untreated as controls. Total RNA was isolated, reverse-transcribed into 32P-labeled cDNA, and hybridized to Atlas human cancer cDNA expression arrays. The values are expression ratios (protein I/II / control).
OA patient | ||||
|---|---|---|---|---|
Protein product of gene | GenBank accession no. | 1 | 2 | 3 a |
Up-regulated | ||||
IL-6 | X04602 | 3.0 | 3.8 | 6.8 |
Interferon-gamma receptor β subunit | U05875 | 5.8 | - | 7.7 |
Interferon regulatory factor 1 | X14454 | - | 11.0 | 5.5 |
Leukemia inhibitory factor | X13967 | 4.1 | - | 5.0 |
Matrix matalloproteinase 3 | X05232 | - | - | 13.3 |
Placenta growth factors 1+2 | X54936 | - | - | 4.6 |
Glia maturation factor β | M86492 | - | - | 4.5 |
Transforming growth factor β2 | M19154 | - | - | 4.5 |
Growth inhibitory factor | D13365 | - | - | 3.4 |
Rho-related GTP-binding protein RhoE | X95282 | - | - | 3.2 |
Vascular endothelial growth factor receptor 1 | X51602 | - | - | 3.2 |
Collagen VI α3 subunit | X52022 | - | - | 3.2 |
Early growth response protein 1 | X52541 | - | - | 3.0 |
TRK-T3 | X85960 | - | - | 3.0 |
Down-regulated | ||||
Vimentin | X56134 | - | 0.3 | - |
Transmembrane protein SEX | X87852 | - | 0.3 | - |
Integrin β8 | M73780 | - | 0.3 | - |