Figure 3
From: TLR2 modulates inflammation in zymosan-induced arthritis in mice
TLR2 mediates an inflammatory response in zymosan-induced arthritis (ZIA). (a) Immunohistochemistry of TLR2 expression in synovial membrane sections of WT mice (day 25 ZIA) showed staining in the sublining (sl) of synovial membrane, whereas inflammatory lymphocytes were not stained. (b) Negative control was performed with 0.5% bovine serum albumin. (100× magnification). (c) 99mTc uptake measurement showed an attenuation of the inflammatory response at days 1 and 25 (P < 0.05) in TLR2-/- ZIA mice (n = 5) compared with WT ZIA mice (n = 5). The technetium ratio was measured as detailed in the Materials and methods section. (d) Histological scoring at day 25 of ZIA showed a significant decrease in cell infiltration in TLR2-/- mice (P < 0.02) compared with control mice, whereas cartilage destruction was similar in both groups. (e) Lymph node cell proliferation to 4 and 8 μg/ml zymosan (Zym 4 and Zym 8, respectively). (f) Antibody production against zymosan in zymosan-treated TLR2+/+ and TLR2-/- mice.