Figure 8From: Enumeration and phenotypical analysis of distinct dendritic cell subsets in psoriatic arthritis and rheumatoid arthritisCytokine release and phenotypic changes following pDC exposure to TLR agonst. Synovial fluid (SF) plasmacytoid dendritic cells (pDCs) mature and release interferon alpha (IFN-α) and tumour necrosis factor alpha (TNF-α) at comparable levels with healthy control peripheral blood (PB) pDCs, but only SF pDCs release IL-10. Cytokine release and the maturation status of pDCs purified from healthy control PB (n = 3) and inflammatory arthritis SF (rheumatoid arthritis, n = 2; psoriatic arthritis, n = 1) were assessed after 24-hour incubation with IL-3-supplemented media alone or containing the TLR9 agonist CpG ODN 2216 (3.2 μg/ml). (a) The concentrations of IFN-α, TNF-α and IL-10 in supernatant from cultures was determined by multiplex and ELISA analysis, and was expressed as release per 5 × 104 cells. Squares indicate individual donors. (b) Maturation and co-stimulation marker expression was determined by flow cytometry, and one representative example from each of the healthy control PB and inflammatory arthritis SF groups are shown.Back to article page