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Figure 8 | Arthritis Research & Therapy

Figure 8

From: The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis

Figure 8

Effects of nuclear factor kappa B (NF-κB) inhibitors on S100A8/A9-mediated cytokine production. Monocytes (1 × 106 cells per ml in culture medium with 10% fetal calf serum), after two-hour pretreatment with or without the inhibitors of NF-κB activation (1 μM), 1-pyrrolidinecarbodithioic acid (PDTC), and Nα-tosyl-phenylalanylchloromethylketone (TPCK), were stimulated for 24 hours with or without S100A8/A9 (1 or 10 μM) in the presence of polymyxin B. Culture supernatants were measured for tumor necrosis factor alpha (TNF-α) concentrations by enzyme-linked immunosorbent assay. Values are the mean ± standard error of the mean. n, number of samples tested.

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Arthritis Research & Therapy

ISSN: 1478-6362

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