Table 1 PCR primers
Gene | Strand | Sequence | Annealing temp (°C) | MgCl2 (mmol/l) | Product size (bp) | Reference/GenBank accession number |
|---|---|---|---|---|---|---|
Acidic SMase | Forward | 5'-ATCGGCCTTAATCCTGGTGA-3' | 58 | 2.5 | 151 | AF325550 |
| Â | Reverse | 5'-GACTGGACACGGAGAGGGC-3' | Â | Â | Â | Â |
Neutral SMase | Forward | 5'-TTGGCAGTGGCCTCTGTGTG-3' | 58 | 2.5 | 151 | NM031360 |
| Â | Reverse | 5'-AGTCCGCTTAGATGGAGCACC-3' | Â | Â | Â | Â |
GAPDH | Forward | 5'-TGGTCACCAGGGCTGCTTTTA-3' | 60 | 2.5 | 746 | U85042 |
| Â | Reverse | 5'-CGCCTGCTTCACCACCTTCT-3' | Â | Â | Â | Â |
Sox9 | Forward | 5'-GGGCGAGCCGGACCTGAAGAA-3' | 53 | 1.5 | 321 | AF278703 |
| Â | Reverse | 5'-CGCTCCGCCTCCTCCACGAAC-3' | Â | Â | Â | Â |
Col2a1 | Forward | 5'-GCCTCGCGGTGAGCCATGATC-3' | 60 | 1.5 | IIA: 472 | Valcourt and coworkers [30] |
| Â | Reverse | 5'-CTCCATCTCTGCCACGGGGT-3' | Â | Â | IIB: 268 | Â |