Figure 8

The effect of inhibitors of uptake on T cell proliferation in vivo. To test the effect of amiloride on mitogenic and type II collagen (CII)-specific T cell proliferation in vivo, groups of four mice were footpad immunized with CII emulsified in TiterMax adjuvant in the absence (no inhibitor) or presence of 150 μg/mouse amiloride (amiloride), and (a) mitogenic or (b) CII-specific T cell responses of the popliteal lymph node cells were assayed in triplicates 7 days later. Radioactivity incorporation was quantified as counts per minute (cpm) and cpm of cells alone was 797.6 (95% confidence interval from 643.7 to 951.4; n = 35). To show biological variation, mean data and error bars denoting 95% confidence interval are presented.