Figure 1

Example of a calibration experiment for the determination of the ΔΔCt values. The figure shows the standard curve for glucosealdehyde phosphate dehydrogenase (GAPDH) based on the plasmid used for standardization. The regression curve has the function f(x) = -3.9x + 42.5. The dashed lines indicate the 95% confidence intervals. Moreover, two aliquots were prepared containing cDNA from a bursa sample, in addition to the plasmid standard. Upright triangle: one volume of bursa sample. Inverted triangle: two volumes of bursa sample. Note that the linearity of measurement is undisturbed by the added samples and that the detected quantities of GAPDH strictly correlate to the amounts added.