Figure 7

BMP-2 and BMP-7 co-localization with AP in bursa tissue with and without C2C12 cells. Indirect immunofluorescence staining of the topical distribution of (a) bone morphogenetic protein (BMP)-2 (green) and (b) BMP-7 (red) and their local effects on C2C12 cells. The images display objective magnifications (×5) of subsequent frozen sections. (c) A section without cultured C2C12 cells stained directly for alkaline phosphatase (AP). (d) A section seeded with C2C12 cells and cultured for 5 days before staining for AP. Note the co-distribution of AP, both the bursa-derived intrinsic enzyme and the C2C12-derived enzyme, with the BMP deposits within the tissue.