Figure 7
Expression of type 1 interleukin-10 receptor (IL-10R1), macrophage colony-stimulating factor receptor (M-CSFR), and type 1 and 2 tumour necrosis factor receptor (TNFR1/2) in synovial tissue (ST) from patients with rheumatoid arthritis. ST sections were stained with mouse immunoglobulin (Ig) G1 anti-IL-10R1 monoclonal antibody (mAb), rabbit IgG anti-TNFR1 Ab, rabbit IgG anti-TNFR2 Ab, rabbit IgG anti-M-CSFR Ab, mouse IgG1 anti-CD68 mAb, and isotype-matched control Abs, followed by incubation with rhodamine-conjugated goat anti-mouse IgG1 mAb and fluorescein isothiocyanate-conjugated anti-rabbit IgG mAb. Two-color immunofluorescence confocal images were obtained for expression of IL-10R1 (red staining), M-CSFR (red), TNFR1 (green), TNFR2 (green), and CD68 (green). The two images were superimposed, and double-positive cells are shown in yellow. Similar staining patterns were obtained in additional analyses from five ST samples from different patients.