Figure 1
Atorvastatin enhances CD59 expression in hypoxia on endothelial cells. (a) Following culture for 48 hours in 21% O2 (normoxia, open bars) or 1% O2 (hypoxia, filled bars), in the presence of increasing concentrations of atorvastatin, endothelial cell CD59 expression was measured by flow cytometry using the mAb BRIC 229. Bars represent the mean relative fluorescence intensity ± standard error of the mean, derived by dividing the mean fluorescence intensity obtained with test mAb by the mean fluorescence intensity with irrelevant isotype-matched control mAb (n = 4), *P < 0.05, **P < 0.01 compared with untreated controls. (b) Human umbilical vein endothelial cells (HUVEC) cultured for 24 hours in 21% O2 (normoxia, N) or 1% O2 (hypoxia, Hy) were lysed and analysed by immunoblotting for expression of HIF-1α, HIF-2α and α-tubulin as a loading control.