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Table 1 Primers used in the real-time PCR

From: Interactions between IL-32 and tumor necrosis factor alpha contribute to the exacerbation of immune-inflammatory diseases

Human IL-32

Sense

5'-TGAGGAGCAGCACCCAGAGC-3'

 

Antisense

5'-CCGTAGGACTGGAAAGAGGA-3'

Human TNFα

Sense

5'-GTCTCCTACCAGACCAAG-3'

 

Antisense

5'-CAAAGTAGACCTGCCCAGACTC-3'

Human β-actin

Sense

5'-TTCCTGGGCATGGAGTCCT-3'

 

Antisense

5'-AGGAGGAGCAATGATCTTGATC-3'

Mouse TNFα

Sense

5'-CATCTTCTCAAAATTCGAG-3'

 

Antisense

5'-TGGGAGTAGACAAGGTACAACCC-3'

Mouse IL-1β

Sense

5'-CAACCAACAAGTGATATTCTCCATG-3'

 

Antisense

5'-GATCCACACTCTCCAGCTGCA-3'

Mouse IL-6

Sense

5'-CACTTCACAAGTCGGAGGCTTA-3'

 

Antisense

5'-GCAAGTGCATCATCGTTGTTG-3'

Mouse β-actin

Sense

5'-AGAGGGAAATCGTGCGTGAC-3'

 

Antisense

5'-CAATAGTGATGACCTGGCCGT-3'

  1. TNFα, tumor necrosis factor alpha.