Figure 2From: Identification of novel citrullinated autoantigens of synovium in rheumatoid arthritis using a proteomic approachCitrullination of F-actin capping protein α-1 subunit (CapZα-1) in synovial tissues of patients with rheumatoid arthritis. The extracts from synovial tissues from three patients (RA103, 107, and 109) were separated by two-dimensional electrophoresis. Proteins were stained with SYPRO Ruby protein gel stain (left column). After transfer, citrullinated proteins were detected by Western blotting with anti-modified citrulline (anti-MC) antibodies (middle column). The protein spots of CapZα-1 confirmed by Western blotting with anti-CapZα-1 antibodies (right column) are indicated by arrows.Back to article page