Figure 2From: Abnormal insulin-like growth factor 1 signaling in human osteoarthritic subchondral bone osteoblastsEffect of insulin-like growth factor (IGF)-1 on alkaline phosphatase activity in osteoarthitis (OA) osteoblasts. Cells were grown to confluence and incubated overnight in serum free medium. Cells were then exposed to 50 nM 1,25(OH)2D3 in Ham F12/DMEM media containing 2% charcoal-treated FBS and in the presence or not of 50 ng/ml IGF-1 with or without 10 μM PD98059 (PD). Results are expressed as the mean ± SEM of control values without IGF-1 for 6 OA osteoblast preparations.Back to article page