Figure 4
Effect of neutralisation of tumour necrosis factor (TNF)-α and/or interleukin (IL)-1 on cytokine and matrix metalloproteinase (MMP) production in osteoarthritis synovial cells. In these experiments, 2 × 106 cells per well were plated into 4 wells on a 24-well plate in 1 ml of RPMI 1640 supplemented with 10% foetal calf serum. The cells in these 4 wells were left untreated, incubated with the p75 TNF-soluble receptor-immunoglobulin fusion protein etanercept (Enbrel), incubated with a neutralising anti-IL-1β antibody, or incubated with a combination of etanercept and anti-IL-1β, as described in Materials and methods. After incubation for 48 hours, the supernatants were removed for enzyme-linked immunosorbent assay analysis of various cytokines (a) and MMPs (b). The data are expressed as percentage of the production of untreated cells, and the standard error of the mean is given (n = 6–7).