Target template | Polymerase chain reaction primers | Product size (bp) | Annealing temperature (°C) |
---|---|---|---|
GAPDH | 5'-TGGTATCGTGGAAGGACTCAT 5'-GTGGGTGTCGCTGTTGAAGTC | 370 | 53 |
ADAMTS4 | 5'-GTCTGTGTCCAGGGCCGATGC 5'-GCCGCCGAAGGATCTCCAGAA | 541 | 61.8 |
ADAMTS5 | 5'-GCGGATGTGTGCAAGCTGACC 5'-AGTAGCCCATGCCATGCAGGA | 487 | 57.4 |
MMP-1 | 5'-ACAAATCCCTTCTACCCGGAA 5'-GGATCCATAGATCGTTTATAT | 314 | 50.8 |
MMP-3 | 5'-CTTTTGGCGAAAATCTCTCAG 5'-AAAGAAACCCAAATGCTTCAA | 404 | 50 |
MMP-9 | 5'-GGCCTCCAACCACCACCACA 5'-CGCCCAGAGAAGAAGAAAAGC | 400 | 61 |
MMP-13 | 5'-TTCTGGCACACGCTTTTCCTC 5'-GGTTGGGGTCTTCATCTCCTG | 273 | 53 |
TIMP-1 | 5'-CCACCTTATACCAGCGTTAT 5'-CCTCACAGCCAACAGTGTAGG | 282 | 54 |
TIMP-2 | 5'-GTGGACTCTGGAAACGACAT 5'-TCTTCTTCTGGGTGGTGCTCA | 265 | 54 |
TIMP-3 | 5'-GGGAAGAAGCTGGTAAAGGAG 5'-GCCGGATGCAGGCGTAGTGTT | 418 | 54 |
Link protein | 5'-GGAAGCAGAGCAAGCCAAGGT 5'-GAAGGAGGCGATCACAGCATC | 448 | 56.1 |