Patient | Autoantibodies | Without absorption | With absorption |
|---|
1 | Anti-whole P (μg/ml) | 7.243 | 1.435 |
|  | Anti-PC22 (μg/ml) | 3.456 | 1.019 |
|  | Anti-PEX.C22 (μg/ml) | 3.787 | 0.416 |
| Â | Anti-N (U/ml) | 4.083 | 1.950 |
2 | Anti-whole P (μg/ml) | 0.140 | 0.050 |
|  | Anti-PC22 (μg/ml) | 0.070 | 0.042 |
|  | Anti-PEX.C22 (μg/ml) | 0.070 | 0.008 |
| Â | Anti-N (U/ml) | 0.789 | 0.588 |
- Cerebrospinal fluid (CSF) samples (50 μl/well) were incubated in wells of a 96-well flat-bottomed microtiter plate with or without confluent SK-N-MC cells fixed with 1% paraformaldehyde at room temperature for 2 hours. After the incubation, CSF samples were recovered and were examined for anti-whole P, anti-PC22, anti-PEX.C22, and anti-N as described in Materials and methods. Anti-N, anti-neuronal cell antibodies; anti-PC22, antibodies directed against the C-terminal 22-amino acid sequences of ribosomal P protein; anti-PEX.C22, autoantibodies directed against the ribosomal P protein epitopes other than the C-terminal 22-amino acid sequence; anti-whole P, antibodies to the whole ribosomal P proteins.
