| | | | Hybridoma response (SI) |
---|
Compound | HC gp-39 | Sequence | HLA-DR binding IC50 (μM) | 5G11 | 8B12 | 14G11 | 20H5 |
---|
1 | 263–275 | RSFTLASSETGVG | 0.08 |
17
|
39
|
30
|
5
|
10 | R263A1 | ASFTLASSETGVG | 0.10 |
23
|
42
|
28
|
5
|
11 | S264A2 | RAFTLASSETGVG | 0.10 |
21
|
43
|
32
|
5
|
12 | F265A3 | RSATLASSETGVG | >35 | 2 |
6
| 2 |
4
|
13 | T266A4 | RSFALASSETGVG | 0.06 |
7
|
14
| 1 |
5
|
14 | L267A5 | RSFTAASSETGVG | 0.08 |
4
| 1 | 1 |
4
|
15 | A268D6 | RSFTLDSSETGVG | 0.24 | 1 | 1 | 1 | 2 |
16 | S269A7 | RSFTLAASETGVG | 0.05 | 2 | 1 | 1 | 2 |
17 | S270A8 | RSFTLASAETGVG | 0.05 |
21
|
29
|
27
|
5
|
18 | E271A9 | RSFTLASSATGVG | 0.04 | 1 | 1 | 1 | 2 |
19 | T272A10 | RSFTLASSEAGVG | 0.04 | 1 |
4
| 1 | 1 |
20 | G273A11 | RSFTLASSETAVG | 0.05 |
7
|
32
|
6
|
5
|
21 | V274A12 | RSFTLASSETGAG | 0.06 |
26
|
40
|
30
|
5
|
22 | G275A13 | RSFTLASSETGVA | 0.05 |
17
|
30
|
20
|
5
|
23 | A3/A8 | RSATLASAETGVG | >36 | ND | ND | ND | ND |
- Binding of peptides to HLA-DR4 (B1*0401) was determined in a competition binding assay. IC50 = 50% inhibitory concentration. IC50 < 1 μM = high affinity binder; IC50 between 1 and 10 μM = good binder; IC50 between 10 and 100 μM = intermediate binder. The HLA-DRB1*0401 expressing B lymphoblastoid cell line was used as a source of antigen-presenting cells. Background values for hybridomas 5G11, 8B12, 14G11 and 20H5 were 17,238, 8,773, 10,131 and 290,666 mean fluorescence units/counts, respectively. The standard deviation of measurements did not exceed 15%. Hybridoma IL-2 response is expressed as stimulation index (SI) calculated as the ratio of mean fluorescence counts of antigen stimulated cultures and control cultures. Values in bold are considered positive (SI > 2). Data are representative of at least three independent experiments. ND, not determined.