| | | | | Concentration (pg/ml) |
---|
Compound | HC gp39 | Sequence | [Peptide] (μg/ml) | Prol (SI) | MIP1α | RANTES | IFNγ |
---|
1 | 263–275 | RSFTLASSETGVG | 10 |
8
|
252
|
23
|
16
|
| | | 2 |
6
|
128
|
13
| 6 |
| | | 0.4 |
3
| 25 | 6 | <5 |
12 | F265A3 | RSATLASSETGVG | 10 |
3
| 27 | 9 | <5 |
| | | 2 | 1 | <24 | <6 | <5 |
13 | T266A4 | RSFALASSETGVG | 10 |
7
|
116
|
18
|
9
|
| | | 2 |
4
|
47
|
11
| <5 |
14 | L267A5 | RSFTAASSETGVG | 10 |
7
|
295
|
32
|
14
|
| | | 2 |
6
|
112
|
11
| <5 |
15 | A268D6 | RSFTLDSSETGVG | 10 |
3
| 29 | <6 | <5 |
| | | 2 | 1 | <24 | <6 | <5 |
16 | S269A7 | RSFTLAASETGVG | 10 | 2 | <24 | <6 | <5 |
| | | 2 | 2 | <24 | <6 | <5 |
17 | S270A8 | RSFTLASAETGVG | 10 |
7
|
279
|
20
|
12
|
| | | 2 |
5
|
86
|
12
| <5 |
18 | E271A9 | RSFTLASSATGVG | 10 | 1 | <24 | <6 | <5 |
| | | 2 | 1 | <24 | <6 | <5 |
19 | T272A10 | RSFTLASSEAGVG | 10 | 1 | <24 | <6 | <5 |
| | | 2 | 1 | <24 | <6 | <5 |
20 | G273A11 | RSFTLASSETAVG | 10 |
4
|
47
|
15
| <5 |
| | | 2 | 1 | <24 | <6 | <5 |
| | No peptide | 0 | 1 | <24 | <6 | <5 |
- Antigen-specific responses (proliferation and cytokine production) were assessed at 96 h. Proliferation is expressed as stimulation index (SI; antigen specific cpm/background cpm). Background was 407 cpm. The standard deviation of measurements did not exceed 30%. For the proliferative assay an SI > 2 (bold) is regarded as positive. Cytokine production of Mip1α, RANTES and IFNγ is expressed in pg/ml. The limit of detection for these cytokines is 24, 5 and 6 pg/ml respectively. Values in bold are regarded as positive. Other cytokines assayed (such as IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p40, IL-12p70, IL-17, granulocyte colony-stimulating factor, granulocyte-macrophage colony-stimulating factor, tumor necrosis factor-α and cytokine-induced neutrophil chemoattractant (KC)) were not detected. Prol, proliferation.