Table 2 PCR primer and probe sequences and efficiencies
Target | Forward primer | Probe | Reverse primer | Efficiency (percent) |
|---|---|---|---|---|
18s | PDAR | PDAR | PDAR | 99.65 |
IL-1β | 5' CGG CCA CAT TTG GTT CTA AGA 3' | 5' ACC CTC TGT CAT TCG CTC CCA CA 3' | 5' AGG GAA GCG GTT GCT CAT C 3' | 90.5 |
TNF α | 5' TGG TGG TCT TGT TGC TTA AAG TTC 3' | 5' TCC CCT GCC CCA ATC CCT TTA TTA CCC G 3' | 5' CGA ACA TCC AAC CTT CCC AAA C 3' | 90.1 |
IL-1 RI | 5' ATT TCT GGC TTC TAG TCT GGT GTT C 3' | 5' ACT TGA TTT CAG GTG AAT AAC GGT CCC C 3' | 5' AAC GTG CCA GTG TGG AGT GA 3' | 98.5 |
TNF RI | 5' CCT GGC CCC AAA CCC AAG 3' | 5' TTC AGT CCC ACT CCA GGC TTC ACC C 3' | 5' GTA TAG GTG GAG CTG GAG GTG 3' | 93.8 |