Figure 3

TauCl inhibited IκBα degradation as potently as did a NF-κB inhibitor (MG132). Synovial cells (5 × 10⁵ cells/60 mm dish/2 ml serum-free media) were treated with taurine chloramine (TauCl) or MG132 30 min before IL-1β (10 ng/ml) stimulation for 30 min. At a concentration of 800 μmol/l, TauCl inhibited the degradation of inhibitor of nuclear factor-κB (IκB)α just as potently as did 1 μmol/l MG132. Three independent experiments were performed with cells from two patients. NF-κB, nuclear factor-κB.