Figure 5
IL-23-induced IL-17 production by activated human T cells in a dose-dependent manner. (a) Human CD3-positive T cells were cultured in the absence or presence of recombinant human (rh)IL-23 (1.0 or 3.0 ng/ml). After 48 hours, secretion of IL-17 in the cell culture supernatant was assayed by ELISA. Results for one representative donor are presented here and are expressed as means and SD. (b) ELISA measurement of IL-17 production by human CD3-positive T cells treated for 48 hours with various concentrations of rhIL-23 (1.0, 3.0, or 10 ng/ml) in the presence or absence of plate-bound anti-CD3 and anti-CD28, for five representative donors. Data are expressed as means and SD. #P = 0.009 versus non–activated T cells (Mann–Whitney U test). (c) ELISA measurement of IFN-γ production by human CD3-positive T cells treated for 48 hours with various concentrations of rhIL-23 (1.0, 3.0, or 10 ng/ml) in the presence or absence of plate-bound anti-CD3 and anti-CD28, for four representative donors. Data are expressed as means and SD. #0.02 versus non–activated T cells (Mann–Whitney U test). (d) Ratio of the production levels of IL-17 to those of IFN-γ (IL-17/IFN-γ) calculated from levels of IL-17 or IFN-γ produced from activated CD3-positive T cells at each concentration of rhIL-23 (0, 1, 3, or 10 ng/ml).