Figure 1

pFAK expression pattern in RA, OA and normal donor (ND) ST. (a) Rheumatoid arthritis synovial tissue (RA ST) stained with anti-pFAK, showing positive staining of the lining cell layer (black arrow) and subsynovial MΦs (white arrowhead) (×200). (b) Positive staining in osteoarthritis (OA) ST (×200). (c) Low pFAK reactivity in normal ST lining (arrow) and subsynovial macrophages (arrowhead). (d) The quantification of data obtained from a, b and c. Bars represent the mean and SEM. Inflam, inflammatory score; Vasc, vascularity score; Lining, ST lining cell layer; Mac, subsynovial MΦs. *P < 0.05. n, numbers of patients. RA ST fibroblasts were stimulated with (e) tumor necrosis factor-α (TNF-α) (10 ng/ml) or (f) interleukin-1β(IL-1β) (10 ng/ml) from 0–120 min. Cell lysates were examined by western blot analysis for pFAK or FAK expression. The results are representative of three experiments.