RA donor | Anti-CD69 | Anti-CD18 | Anti-CD49d |
---|
 | Inhibition (%) |
P
| Inhibition (%) |
P
| Inhibition (%) |
P
|
---|
1 | 17 | <0.01 | 0 | NS | 0 | NS |
2 | 23 | <0.05 | 22 | <0.01 | 36 | <0.01 |
3 | 22 | NS | 26 | NS | 23 | NS |
4 | ND | NS | 51 | <0.01 | 0 | NS |
- Rheumatoid arthritis (RA) synovial mononuclear cell (MNC) cells were cultured in triplicate with anti-CD69, anti-CD18, or anti-CD49d antibodies at a final concentration of 5 μg/ml for 24 hours at 37°C, as described in Materials and methods. Spontaneous tumour necrosis factor (TNF)-α production was measured in the supernatants by ELISA and results expressed as percentage inhibition relative to the control wells (cells only). Significance (P value) was calculated using one-way analysis of variance with post hoc Bonferroni correction.