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Figure 3 | Arthritis Research & Therapy

Figure 3

From: DNA-like class R inhibitory oligonucleotides (INH-ODNs) preferentially block autoantigen-induced B-cell and dendritic cell activation in vitro and autoantibody production in lupus-prone MRL-Faslpr/lprmice in vivo

Figure 3

The size of the linear overhang determines the potency difference between class R and class B inhibitory oligonucleotides (INH-ODNs). Total mouse B cells were stimulated with 33 nM CpG-1826 together with indicated class R or class B INH-ODNs added simultaneously and used over the concentration range shown. (a) Palindromic Class R INH-4 and linear INH-13 ODNs with CCT/GGG blocks at the 3' were used. (b) Palindromic Class R INH-1 and linear INH-18 ODNs with CCT/GGG blocks at the 5' end were used. (c) INH-ODNs with short, medium or long 3' linear overhangs or linear INH-ODNs of the equal length were used. (d) INH-ODNs with short, medium or long 5' linear overhangs or linear INH-ODNs of the equal length were used. Inhibition of CpG-1826-induced rescue from apoptosis is shown. Sequences of INH-ODNs are shown in Table 1 (n = 3 to 5). *P < 0.05. OVHG, overhang; scr., scrambled.

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Arthritis Research & Therapy

ISSN: 1478-6362

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