Table 1 Percentage of fibroblasts and contaminating cells in primary cultures of RA and OA synovial fibroblast cells at various passages
Passage | Cell type | % positive cells (Mean ± SEM)* | |
|---|---|---|---|
RA SF | OA SF | ||
First | Fibroblast (Prolyl-4-hydroxylase +)1 | 75 ± 8.0 | 68 ± 5.0 |
Monocyte/macrophage (CD14+)2 | 15 ± 2.0 | 21 ± 3.5 | |
T-cells (CD3+)3 | 0.8 ± 0.2 | 1.2 ± 0.3 | |
B-cells (CD20+)4 | 0.9 ± 0.3 | 0.8 ± 0.2 | |
Third | Fibroblast (Prolyl-4-hydroxylase +) | 99 ± 0.5 | 98.5 ± 0.6 |
Monocyte/macrophage (CD14+) | 0.8 ± 0.2 | 0.6 ± 0.1 | |
T-cells (CD3+) | 0.5 ± 0.1 | 0.8 ± 0.2 | |
B-cells (CD20+) | 0.6 ± 0.2 | 0.5 ± 0.1 | |
Fourth | Fibroblast (Prolyl-4-hydroxylase +) | 98 ± 0.4 | 99.2 ± 0.4 |
Monocyte/macrophage (CD14+) | 1.0 ± 0.5 | 0.95 ± 0.3 | |
T-cells (CD3+) | 0.5 ± 0.2 | 0.5 ± 0.1 | |
B-cells (CD20+) | 0.9 ± 0.1 | 0.8 ± 0.1 | |