Figure 5

Hematoxylin and eosin staining of paws from mice that received various treatments. (a) Ankle joint (clinical score 0) from a mouse that received rat IgG control antibody demonstrates a mild inflammatory infiltrate within a non-distorted joint space. A mild degree of synovial hyperplasia is also present. No significant cartilage or bone destruction is seen (hematoxylin and eosin, 20 ×). (b) Arthritic joint (clinical score 4) from a mouse that received anti-IFN-γ antibody, demonstrating inflammatory cells, with partial filling of the joint space. Mild synovial hyperplasia is present along with early, minimal alteration of cartilage. Inflammatory changes extend into the adjacent soft tissue. No significant bony changes are present and the joint space is otherwise intact (hematoxylin and eosin, 10 ×). (c) Arthritic joint (clinical score 4) from a mouse that received neutralizing antibodies to IFN-γ + IL-4, demonstrating severe inflammatory changes including complete filling of the joint space and extension to the soft tissue. Cartilage is significantly destroyed and the bone shows a substantial amount of destruction and remodeling (hematoxylin and eosin, 10 ×).