Table 4 PCR sensitivities of the different DNA extraction methods detection Chlamydia trachomatis EB and C. trachomatis PBMO DNA/ml SF using PCR-system 1 for amplification immediately after extraction and post storage at -20°C for four months
Sensitivity achieved with PCR amplification system 1 | 1 Alkaline lysis immediately | 1 Alkaline lysis ps | 2 Qiaex II gel extraction kit® +CTAB immediately | 2 Qiaex II gel extraction kit® + CTAB ps | 4 Qiagen Tissue Kit® immediately | 4 Qiagen Tissue Kit® Ps | 5 Qiagen Stool Kit® immediately | 5Qiagen Stool Kit® ps |
|---|---|---|---|---|---|---|---|---|
(C. trachomatis EB/ml SF) | (0.1 -10) M 1 | 10 (1-10) M 10 | 0.1 | 1000 (1000) M 1000 | 1 (1-10) M 1 | 10 (1-10) M 10 | 1 (1-100) M 100 | 1 (1-10) M 10 |
Statistical analysis ps | See Table 3 | * | See Table 3 | * | See Table 3 | See Table 3 | ||
(C. trachomatis PBMO/ml SF) | (0.1-1) M 0.1 | 1000 (1000) M 1000 | 10 (0.1 - 100) M 10 | 1000 (1000) M 1000 | 10 (1-100) M 10 | 1000 (1000) M 1000 | 1 (0.1-1) M 1 | 1000 (1000) M 1000 |
Statistical analysis ps | See Table 4 | * | See Table 4 | * | See Table 4 | * | See Table 4 | * |