Figure 4

Autophagy protects against ER stress in RASF. OASF and RASF were transfected with non-specific or beclin siRNA, followed by thapsigargin treatment for 60 h. Non-specific and beclin siRNA were transfected into OASF and RASF. Sixteen hours later, total protein was extracted. SDS-PAGE and immunoblotting were performed with anti-beclin or actin antibody (a). Autophagosomes (b) and dead cells (c) were counted as previously described. Data represent means ± S.E. (n = 6). *P < 0.05, versus non-specific siRNA-transfected OASF with thapsigargin. ^#P < 0.05, versus beclin siRNA-transfected OASF with thapsigargin.