Figure 3

Alteration of cytokine and chemokine levels after OAZ silencing. a. IFN-γ, IL-10, IL-12 and IL-21 levels were significantly decreased in culture supernatants of OAZ silence group (silence) as compared to those of negative control group (control), while IL-4 levels remained unchanged and CCL2 levels were increased in culture supernatants of silence group. Values were shown as means ± SEM (** P < 0.01, * P < 0.05). b, c. The relationship between OAZ expression and IL-21 as well as CCL2. There was a tendency for the excitation-inhibition ratio (EIR) of OAZ expression to be positively correlated with EIR of IL-21 level (Pearson r = 0.83, P = 0.08), while the EIR of OAZ expression was negatively correlated with EIR of CCL2 level (Pearson r = -0.89, P < 0.05). d, e. Association of IL-21, CCL2 and ANA level. EIR of IL-21 level was positively correlated with EIR of ANA value (Pearson r = 0.99, P < 0.01), while EIR of the CCL2 level was negatively correlated with EIR of ANA value (Pearson r = -0.98, P < 0.01).