Figure 3

Overexpression of miR-146a suppresses T cell apoptosis. (a) The expression of miR-146a in Jurkat T cells measured by quantitiative RT-PCR increased more than 30 fold after transfection with FUGW-miR-146a, but showed no change after transfection with the control vector FUGW-FF3. (b) After phytohemagglutinin (PHA) stimulation, Jurkat T cells with miR-146a overexpression showed a similar proliferative response as their controls. (c) Upon anti-Fas antibody treatment for six hours, miR-146a-overexpressing Jurkat T cells displayed significantly reduced apoptosis as detected by flow cytometry. (d) After six hours of anti-Fas antibody treatment, apoptotic Jurkat T cells were evaluated. The data are derived from three independent experiments (mean ± standard deviation). **P < 0.01.