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Table 1 Description of the sequences used to quantify gene expression and real-time reaction efficiencies of PCR assays

From: Biomechanical modulation of collagen fragment-induced anabolic and catabolic activities in chondrocyte/agarose constructs

Gene

Gene ID

Sequences

Product size (bp)

Efficiency

MMP-3

    
 

396769

Forward: 5'-ACCCAAGAAGTATCCACACCCT-3'

215

1.98 ± 0.06

  

Reverse: 5'-TGCTTCAAAGACAGCATCCACT-3'

  

MMP-13

    
 

397346

Forward: 5'-CCAAAGGCTACAACTTGTTTCTTG-3'

77

1.99 ± 0.03

  

Reverse: 5'-TGGGTCCTTGGAGTGGTCAA-3'

  

Collagen type II

    
 

397323

Forward: 5'-CGCTGAACATCCTCACAAC-3'

249

1.98 ± 0.19

  

Reverse: 5'-TCCTGTAGATACGCCTAAGC-3'

  

Fibronectin

    
 

397620

Forward: 5'-GACAGATGAGCTTCCCCAAC-3'

752

2.02 ± 0.09

  

Reverse: 5'-CACTGCCAAAGCCTAAGCAC-3'

  

GAPDH

    
 

396823

Forward: 5'-AATCCCATCACCATCTTCCA-3'

318

2.03 ± 0.01

  

Reverse: 5'-TGTGGTCATGAGTCCTTCCA-3'

  
  1. Primers used in quantitative polymerase chain reaction (qPCR) experiments with SYBR green produced amplicons of 77 to 752 base pairs with efficiency values between 1.98 and 2.03. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MMP-3, matrix metalloproteinase-3; MMP-13, matrix metalloproteinase-13.
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Arthritis Research & Therapy

ISSN: 1478-6362

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