Figure 1From: Effects of inflammatory cytokine IL-27 on the activation of fibroblast-like synoviocytes in rheumatoid arthritisExpression of functional IL-27 heterodimeric receptor on FLS. (A, B). Total RNA was extracted from control FLS, RA-FLS and PBMC (positive control), followed by quantitiatve real time RT-PCR analysis for gp130, WSX-1 and GAPDH (house keeping gene) expression. (C) Representative histograms of cell surface expression of gp130 on control FLS, RA-FLS and human CD4+T cells (positive control) determined by flow cytometry. (D) Representative Western blot analysis of WSX-1 protein expression of control FLS, RA-FLS and PBMC (positive control). GAPDH was used as protein control to ensure an equal amount of loaded protein. All the experiments were performed in three independent replicates.M, 100 base-pair molecular size marker; PBMC, peripheral blood mononuclear cells.Back to article page