W.L | ABSORBANCE | Â |
nm | Â | Â |
 | The precipitated |  | The protein-free |  |
 | protein solution |  | supernatant. |  |
 | B0 | B24 | B0-24 | B72 | A0 | A2 | A0 - A2 |
270 | 0.620 | 0.600 | 0.020 | 0.63 | 0.210 | 0.160 | 0.050 |
275 | 0.620 | 0.620 | 0.000 | 0.64 | 0.210 | 0.155 | 0.055 |
280 | 0.610 | 0.600 | 0.010 | 0.65 | 0.220 | 0.150 | 0.070 |
283 | 0.600 | 0.570 | 0.030* | 0.65 | 0.230 | 0.145 | 0.085 |
285 | 0.580 | 0.570 | 0.010 | 0.63 | 0.250 | 0.140 | 0.110 |
290 | 0.520 | 0.510 | 0.010 | 0.55 | 0.270 | 0.140 | 0.130 |
293 | 0.480 | 0.470 | 0.020 | 0.52 | 0.280 | 0.140 | 0.140* |
295 | 0.400 | 0.380 | 0.020 | 0.42 | 0.270 | 0.135 | 0.135 |
300 | 0.300 | 0.290 | 0.010 | 0.40 | 0.230 | 0.130 | 0.100 |
- The precipitated-protein soution: B0 : The asorbance before the addition of uricase. B24 : The absorbance after 24 hours incubation with uricase. B0-B24 : The decrease in the absorbance after incubation for 24 hours with uricase, the max. decrease is at 283 nm. B72 : The absorbance after 72 hours incubation with uricase, the absorbance recorded was higher than that recorded before the action of uricase over the whole region of measurement (270-300 nm). The protein -free supernatant : A0 : The absorbance before the addition of uricase. A2 : The absorbance two hours after the action of uricase. A0 - A2 : The difference between absorbance before and after two hours incubation with uricase, the max. decrease reached at 293 nm.